Hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury

A technology of hydrangea and polysaccharide, applied in the field of medicine, can solve the problems of unclear pharmacodynamic material basis, unclear anti-myocardial ischemia-reperfusion injury and other problems, and achieve the effect of reducing myocardial ischemia-reperfusion injury.

Active Publication Date: 2022-01-14
赵顺英
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Preliminary studies have found that the hydrangea extract has anti-myocardial ischemia-reperfusion injury, but the material basis of the drug effect is still unclear. It is not clear whether the polysaccharides in hydrangea have anti-myocardial ischemia-reperfusion injury

Method used

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  • Hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury
  • Hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury
  • Hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: extraction and purification experiment

[0023] Collect Hydrangea macrophylla leaves, wash, dry in the shade, crush, take 1kg of powder, add 10L deionized water, heat reflux extraction 3 times, 1.5h each time, combine the extracts, filter, concentrate under reduced pressure to 3L, in 5000r / / Centrifuge at a speed of 1 min for 15 min, collect the supernatant, add three times the volume of absolute ethanol, mix well, let stand for 12 h, collect the precipitate, redissolve with 3L deionized water, centrifuge at 5000r / min for 15 min, and collect the supernatant , repeated alcohol precipitation once according to the same method, collected the precipitate, washed with absolute ethanol and acetone, redissolved in deionized water, deproteinized by Sevag method, and freeze-dried.

[0024] Finally, 21.8 g of polysaccharides were collected, and the purity of the polysaccharides determined by the phenol-sulfuric acid method was 92.5%.

Embodiment 2

[0025] Embodiment 2: pharmacological activity experiment

[0026] 1. Materials and methods

[0027] 1. Animal grouping, modeling and administration

[0028] Thirty adult male SD rats (body weight 260-300 g) were randomly divided into sham operation group, myocardial ischemia-reperfusion model group and hydrangea polysaccharide group, with 10 rats in each group. Myocardial ischemia-reperfusion model was produced by ligation of the left anterior descending coronary artery commonly used in the field, and the specific operations were as follows:

[0029] Rats were anesthetized with 3% pentobarbital sodium, fixed, and electrodes were inserted subcutaneously in the limbs to monitor lead II electrocardiogram, intubated, and connected to the animal ventilator; the distance between the 3rd and 4th intercostal space on the left side of the rat was about 0.5cm from the left edge of the sternum Make an incision, bluntly dissect the chest wall layer by layer, ligate the left anterior des...

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Abstract

The invention discloses hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury. The invention provides a hydrangea polysaccharide, which is prepared by the following method: collecting hydrangea macrophylla leaves, washing, drying in the shade, pulverizing, extracting with deionized water under hot reflux, filtering, concentrating the filtrate under reduced pressure, and centrifuging to collect the supernatant, Add three times the volume of absolute ethanol, mix well, let stand for alcohol analysis, collect the precipitate, redissolve with deionized water, repeat the same method of centrifugation, alcohol precipitation once, collect the precipitate, wash with absolute ethanol and acetone, and deionized water Reconstituted, deproteinized by Sevag method, and freeze-dried. Pretreatment with the hydrangea polysaccharide can effectively reduce myocardial ischemia-reperfusion injury in rats, and its mechanism of action is related to reducing myocardial cell apoptosis in ischemia-reperfusion rats, and can be used to prepare drugs for antagonizing myocardial ischemia-reperfusion injury .

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to hydrangea polysaccharide and its application in treating myocardial ischemia-reperfusion injury. Background technique [0002] As a complex physiological and pathological process, myocardial ischemia-reperfusion injury has always been the focus of attention in the cardiovascular field. In recent years, it has been reported in the literature that when myocardial ischemia-reperfusion injury occurs, the way of drug pretreatment can protect the myocardial tissue of ischemia-reperfusion, which may be related to the drug activation of the body's endogenous protective mechanism or the release of endogenous protective mediators. . [0003] Hydrangea macrophylla (Thunb.) Ser. is a deciduous shrub growing in the south of the Yangtze River in my country. Its whole herb is commonly used in the folk to treat diseases such as dysentery, heart heat and palpitations. Modern pharmacological a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P9/10
CPCC08B37/0003A61K31/715A61P9/10
Inventor 吴海靖其他发明人请求不公开姓名
Owner 赵顺英
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