Method for optimizing water extraction of gypenoside by using response surface method

An extraction method and a technology of response surface methodology, which are applied in the field of optimizing the extraction of total saponins from small blue powder by using response surface methodology, can solve the problems of organic reagent pollution, disconnection between theoretical research and practical application, etc., so as to avoid the discharge and extraction of pollutants. The process is accurate and reliable, and the effect of reducing energy consumption

Inactive Publication Date: 2020-02-18
平利县神草园茶业有限公司
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Problems solved by technology

[0003] Gyp is favored by researchers because of its anti-tumor, anti-oxidation, anti-aging, anti-inflammatory and immune regulation and other biological activities. According to the principle of similar compatibility, scholars mainly extract Gyp with ethanol reflux, such as Chinese patent CN201810458420. 2 announced a method for extracting gypenosides, including crushing of medicinal materials, ethanol extraction and macroporous resin purification, wherein ethano

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  • Method for optimizing water extraction of gypenoside by using response surface method
  • Method for optimizing water extraction of gypenoside by using response surface method
  • Method for optimizing water extraction of gypenoside by using response surface method

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[0024] In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and specific embodiments of the description. Utilize a kind of superfine powder of Gynostemma pentaphyllum to obtain by superfine powder process, the extraction method that utilizes response surface methodology to optimize the total saponins of small blue powder provided by the present embodiment is carried out in the following manner:

[0025] In the first step, a sample solution is prepared.

[0026] Accurately weigh 1 g of small blue powder, add water for leaching, centrifuge at 5000 rpm for 10 min, collect the supernatant for later use, measure and calculate the total saponin content in the extract according to the vanillin-glacial acetic acid method.

[0027] In the second step, the vanillin-glacial acetic ac...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for optimizing water extraction of gypenoside by using a response surface method. The method specifically comprises the following steps: (1) preparing a gynostemma pentaphylla sample solution; (2) measuring the content of saponin in the sample solution by using a vanillin-glacial acetic acid method; (3) performing test design and statistical analysis; and (4) performing data process. The method provided by the invention has the advantages of high yield, accurate and reliable extraction process and the like, is simple and feasible, and has low extraction cost. Through adoption of the response surface method, scientificity of an optimal extraction condition is ensured, and good economic benefits and social benefits can be achieved.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for optimizing the extraction of total saponins from blue powder by using a response surface method. Background technique [0002] Gynostemma pentaphyllum is a perennial herbaceous vine of the Cucurbitaceae Gynostemma genus. It was first recorded in the "Famine Rescue Materia Medica" in the Ming Dynasty. In addition, it was also included in "Chinese Materia Medica", "Chinese Medicine Dictionary" and "National Chinese Herbal Medicine Collection". Gynostemma has anti-inflammatory and detoxification, cough and expectorant effects, and it is mostly used in the folk treatment of chronic bronchitis, infectious hepatitis, nephritis and gastroenteritis. Modern pharmacological studies have proved that the saponin (Gyp) contained in Gynostemma pentaphyllum is its main active ingredient. Gyp has a tetracyclic triterpene dammar-type structure similar to ginsenoside, and Gy...

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Application Information

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IPC IPC(8): A61K36/424A23F3/34G01N21/31
CPCA23F3/34A61K36/424A61K2236/333G01N21/3103
Inventor 刘全宏王布雷殷刚
Owner 平利县神草园茶业有限公司
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