Method for optimizing water extraction of gypenoside by using response surface method

Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for optimizing water extraction of gypenoside by using a response surface method. The method specifically comprises the following steps: (1) preparing a gynostemma pentaphylla sample solution; (2) measuring the content of saponin in the sample solution by using a vanillin-glacial acetic acid method; (3) performing test design and statistical analysis; and (4) performing data process. The method provided by the invention has the advantages of high yield, accurate and reliable extraction process and the like, is simple and feasible, and has low extraction cost. Through adoption of the response surface method, scientificity of an optimal extraction condition is ensured, and good economic benefits and social benefits can be achieved.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for optimizing the extraction of total saponins from blue powder by using a response surface method. Background technique [0002] Gynostemma pentaphyllum is a perennial herbaceous vine of the Cucurbitaceae Gynostemma genus. It was first recorded in the "Famine Rescue Materia Medica" in the Ming Dynasty. In addition, it was also included in "Chinese Materia Medica", "Chinese Medicine Dictionary" and "National Chinese Herbal Medicine Collection". Gynostemma has anti-inflammatory and detoxification, cough and expectorant effects, and it is mostly used in the folk treatment of chronic bronchitis, infectious hepatitis, nephritis and gastroenteritis. Modern pharmacological studies have proved that the saponin (Gyp) contained in Gynostemma pentaphyllum is its main active ingredient. Gyp has a tetracyclic triterpene dammar-type structure similar to ginsenoside, and Gy...

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Problems solved by technology

[0003] Gyp is favored by researchers because of its anti-tumor, anti-oxidation, anti-aging, anti-inflammatory and immune regulation and other biological activities. According to the principle of similar compatibility, scholars mainly extract Gyp with ethanol reflux, such as Chinese patent CN201810458420. 2 announced a method for extracting gypenosides, including crushing of medicinal materials, ethanol extraction and macroporous resin purification, wherein ethanol extraction is the key step, however, the introduction of ethanol increases the production cost of saponins on the one hand, and on the other hand On the one hand, it also brings pollution of organic reagents, and the current development of Gynostemma pentaphyllum resources is mainly based on tea beverages. The essence of the tea soup is the water extract of Gynostemma pentaphyllum. There is a big disconnect between theoretical research and practical application.

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