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Culture medium for tissue culture of hedysarum scoparium

A technology of tissue culture and culture medium, applied in the field of plant biology, to achieve the effect of increasing yield

Active Publication Date: 2020-02-21
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the tissue culture of flower sticks is still in the exploratory stage in the existing technology, and there are not many materials that can be referred to when formulating a technical plan, and all of them must be solved one by one through experiments. Selection of medium, culture conditions and rapid propagation technology after seedling emergence, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0028] Example 2. Screening of the best medium for each stage of flower stick tissue culture

[0029] Take B5 as the basic medium, use 3.5g / L carrageenan as the solidifying agent, replace sucrose with 40g / L edible sugar as the carbon source, use tap water instead of distilled water, and add different ratios of cytokinin and auxin to the culture medium filter.

[0030] 1. Selection of primary induction medium

[0031] Set the concentration of 6-BA to two concentration gradients of 0.2mg / L and 0.5mg / L, the concentration of NAA to two concentration gradients of 0.01mg / L and 0.05mg / L, and the concentration of GA3 to 0.1mg / L and 0.05mg / L two concentration gradients, the concentration of CH is set to two concentration gradients of 0.5mg / L and 1mg / L, and different combinations are made. When inoculating, each bottle is connected with 2-3 flower stick growth points (about 0.2-0.5cm in length) , to observe the occurrence of adventitious buds on different media.

[0032] The conditi...

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Abstract

The invention provides a culture medium for tissue culture of hedysarum scoparium. The culture medium comprises a primary induction medium, a subculture medium and a rooting medium. Growth points on young shoots of the hedysarum scoparium can be used as explants, and primary induction culture, subculturing, rooting culture and transplanting are carried out after sterilization. The culture medium has the advantages that the selected optimal culture medium formula and combinations thereof for each growth stage of the whole tissue culture process of the hedysarum scoparium can fully meet nutrition requirements and growth and development requirements in each stage of the hedysarum scoparium, the whole process of tissue culture and expanding propagation of the hedysarum scoparium is promoted, the successful implementation of the tissue culture of the hedysarum scoparium can be ensured, and a strong support is provided for large-scale production of the hedysarum scoparium. The culture mediumhas the advantages of simple formula, low cost and good application prospect.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a culture medium for each stage in the tissue culture of flower sticks. The present invention screens out the optimal culture medium used in different stages of flower stick tissue culture, determines the ratio of each hormone in the culture medium, and the culture medium formulated according to the formula of the present invention can meet the nutritional needs and growth and development. Background technique [0002] Flower sticks, also known as twig astragalus, huazichai, huachai, flower seedlings, flower caps and oxtail tips, etc., are large deciduous shrubs of the genus Astragalus in the Fabaceae Papilionaceae subfamily, and are typical sand-fixing pioneer plants in desert areas. Distributed in the arid and semi-arid deserts or sandy lands of Central Asia, China's Gurbantunggut Desert, Badain Jaran Desert, Hexi Corridor Sandy Lands, Tengger Desert, Ulan Buhe D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001Y02A40/22
Inventor 马怀林贾振宇刘思泱高秀梅田志来白俊梅智磊崔海鹏王媛媛张跃华潘亮荆莹
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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