A monoclonal antibody, coupling protein, epitope peptide and hybridoma cell line for detecting human cd117 protein
A hybridoma cell line, monoclonal antibody technology, applied in the field of immunology, can solve problems such as high cost
Active Publication Date: 2021-08-31
河南赛诺特生物技术有限公司
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AI Technical Summary
Problems solved by technology
Most of the anti-CD117 monoclonal antibodies used in China come from foreign imported antibodies (clone numbers: YR145, EP10, A4502), which are expensive; in order to obtain anti-CD117 monoclonal antibodies with comparable or excellent effects to imported reagents and reduce the burden on patients, To reduce the cost of diagnosis and treatment, it is of great significance to develop an anti-CD117 monoclonal antibody with clinical application
Method used
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Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0036] The amino acid sequence of the epitope peptide in this example is NRQKPVVDHSVRINS. The sequence is designed according to the P10721 protein sequence published by Uniprot, which is the core polypeptide sequence of the CD117 protein epitope.
Embodiment 2
[0038]The amino acid sequence of the epitope peptide in this example is CSPNRQKPVVDHSVRINS. The epitope peptide is based on the core polypeptide sequence in Example 1 with a "CSP" auxiliary sequence added at the N-terminus, and the epitope peptide can be directly synthesized by chemical methods.
Embodiment 3
[0040] The human CD117 coupling protein in this example includes two parts, a synthetic human CD117 epitope peptide and a carrier protein. The synthetic human CD117 antigen epitope peptide sequence is CSPNRQKPVVDHSVRINS; the carrier protein is KLH (hemocyanin).
[0041] The human CD117 coupling protein in this example was synthesized and coupled by a biological company.
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Abstract
The invention relates to a monoclonal antibody, a coupling protein, an antigenic epitope peptide and a hybridoma cell line for detecting human CD117 protein, and belongs to the technical field of immunology. In the present invention, the polypeptide sequence with NRQKPVVDHSVRINS as the core is selected as the epitope peptide to prepare monoclonal antibodies. In order to improve the immune effect of the epitope peptide, at least 1 and no more than 10 are added to the N-terminal or C-terminal of the aforementioned core sequence Amino acid, so that the N-terminal or C-terminal is an amino acid containing a sulfhydryl group; the sulfhydryl group on the epitope peptide is coupled with the amino group on the carrier protein through a coupling agent to obtain the human CD117 antigen, which is used to immunize animals to obtain human CD117 Monoclonal antibodies. The present invention provides a new way for the domestic production of human CD117 antibody, and the obtained monoclonal antibody has high specificity and sensitivity and is cheap.
Description
technical field [0001] The invention relates to a monoclonal antibody, a coupling protein, an antigenic epitope peptide and a hybridoma cell line for detecting human CD117 protein, and belongs to the technical field of immunology. Background technique [0002] CD117 protein is a glycosylated phosphoprotein with a molecular weight of 145kDa. It has a transmembrane region, the amino terminal is located outside the cell membrane, and the carboxy terminal is located inside the plasma membrane. The CD117 protein has five immunoglobulin-like domains and a type III tyrosine kinase growth factor domain, and plays an important role in regulating cell survival and proliferation, hematopoiesis, stem cell maintenance, mast cell development, migration and function, and melanogenesis effect. [0003] CD117 protein is expressed in normal tissue cells such as hematopoietic stem cells, melanocytes, mast cells, Cajal cells, germ cells, skin basal cells and mammary ductal epithelium, and in g...
Claims
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IPC IPC(8): C07K16/28C07K19/00C07K14/705C12N5/20
CPCC07K14/70503C07K14/795C07K16/2803C07K2319/40
Inventor 王亚杰徐星星常秋霜洪赟智李森刘玲玲齐华
Owner 河南赛诺特生物技术有限公司