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Method for screening myxobacteria cracking haematococcus from sludge

A technology of Haematococcus and Myxobacteria, which is applied in the field of microorganism screening and achieves the effects of convenient operation, abundant Myxobacteria resources and low cost

Active Publication Date: 2020-02-21
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the method of screening myxobacteria for lysing Haematococcus

Method used

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  • Method for screening myxobacteria cracking haematococcus from sludge
  • Method for screening myxobacteria cracking haematococcus from sludge
  • Method for screening myxobacteria cracking haematococcus from sludge

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Embodiment 1

[0037] A method for screening myxobacteria that cracks Haematococcus from sludge, adding inactivated Haematococcus dropwise on Wax solid medium, adding pretreated sludge around the inactivated Haematococcus for cultivation, and forming fruiting bodies ; The fruiting body is purified and cultured to obtain the myxobacteria that lyse Haematococcus. The specific method is as follows:

[0038] 1. Preservation of algae species and expanded cultivation of algae species:

[0039]1.1 Preparation of BBM medium: Weigh the components of the BBM medium according to Table 1, and dissolve them in 500 mL of water to obtain a BBM medium whose concentration is magnified 100 times. Store the prepared medium in a refrigerator at 4°C. When using, dilute 100 times with pure water according to the configuration medium, divide the package, seal the bottle mouth with treated gauze, and sterilize at 121°C for 30 minutes before use.

[0040] Table 1 BBM medium components

[0041] Element ...

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Abstract

The invention discloses a method for screening myxobacteria cracking haematococcus from sludge. The method comprises the following steps: dropwise adding inactivated haematococcus on a Wax solid medium, and adding the pretreated sludge around the inactivated haematococcus for culturing to form fruiting bodies; and performing purification and culture on the fruiting bodies to obtain the myxobacteria cracking haematococcus. The method for screening myxobacteria cracking haematococcus from sludge has the advantages of simple process, convenient operation, low cost, and the like. The myxobacteriaobtained by screening can crack haematococcus, a new idea for studying haematococcus cracking methods is provided, a foundation for optimizing the method for crakcing haematococcus and extracting astaxanthin from haematococcus is laid, and the application range of myxobacteria is broadened. Meanwhile, the sludge use in the method has wide source distribution, the myxobacteria are screened from thesludge, and thereby the enrichment of myxobacteria resources is facilitated.

Description

technical field [0001] The invention relates to a method for screening microorganisms, in particular to a method for screening myxobacteria that lyse Haematococcus from sludge. Background technique [0002] Myxobacteria (Myxobacteria) is a Gram-negative bacterium that mainly exists in various soils, decaying trees, tree epidermis and animal food waste excrement. Myxobacteria are classified as belonging to the δ branch of Proteobacteria. The myxobacteria discovered today are classified into 3 suborders, and there are 21 genera, including about 50 species. Myxobacteria can produce constituent structures Different and rich metabolites have high research value and are an important microbial resource. The growth and development process of myxobacteria generally includes vegetative cell growth phase and cell development phase. Under nutrient-poor conditions, myxobacterial cells will aggregate and move, and most of the cells will autolyse, while the surviving cells accumulate in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12C12R1/89
CPCC12N1/12C12N1/20
Inventor 陈治国刘臻张晓粤宋学文
Owner CHANGSHA UNIVERSITY