A kind of sporulation culture method of Phytophthora soybean

Abstract

The invention belongs to the technical field of microbes, and specifically discloses a spore-producing culture method for Phytophthora soybean. In the present invention, after the growth of mycelium is completed, the liquid medium is added to the step of oligotrophic shaking culture, so that the bacteria cake is cultivated in 10% liquid V8 medium, and the growth of mycelia in the oligotrophic state will be inhibited, thereby stimulating reproductive growth and facilitating swimming The production of sporangia. Further, through shaking culture in the oligotrophic stage combined with sterilized water washing and soil extract induction, a large number of sporulation of Phytophthora soybean was achieved on V8 medium, and the number of spores reached 2×10 5 Individual / mL, sporulation effect and stability are much higher than the methods disclosed in the prior art.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a spore-forming culture method of Phytophthora soybean. Background technique [0002] Soybean blight (Phytophthora root rot, PRR for short), also known as soybean Phytophthora or Phytophthora soybean root rot, its pathogen is Phytophthora sojae Kaufmann&Gerdemann. The disease is a widely distributed and extremely serious soil-borne disease, and it is also an important foreign quarantine object in my country. The disease was first discovered in Indiana, USA in 1948, and Japan, Australia, Canada and other countries have also reported the occurrence of the disease (Shen Chongyao, 1991; chmittennerA F.1985). Phytophthora sojae was first isolated in Northeast my country in 1989. Since then, many researchers in my country have carried out research on Phytophthora soybean successively (Shen Chongyao, 1991; Zuo Yuhu, 2001; Zuo Yuhu, 2002; Zhu Zhendong, 1999) . Inducing Phy...

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Problems solved by technology

However, although this literature has conducted research on different culture media, times of changing liquids, intervals of changing liquids, culture temperature, light, culture time, nutrients, bacterial age, and mycelium quantity, etc., in general, the experimental results show that CA The sporulation yield of the medium is the highest, while the sporulation yield of V8 medium and Lima bean medium is very low

However, the inventors of the present application verified by repeated tests and found that the sporulation of the CA medium was not stable, and sometimes even failed to produce sporulation, which brought great difficulties to follow-up tests.

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