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Application of SNP Markers in the Prognosis of Pancreatic Cancer

A marker, pancreatic cancer technology, applied in the field of biomedicine, can solve the problems of lack of systematic treatment, hidden onset and uncertainty of pancreatic cancer, etc.

Active Publication Date: 2020-08-11
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pancreatic cancer has an insidious onset, difficulty in early diagnosis, distant metastasis, and resistance to chemotherapy. Most pancreatic cancer patients are in the middle and late stages when they are discovered, and lose the chance of radical cure. Due to the rapid proliferation ability and high tendency of invasion and metastasis of pancreatic cancer, the pathogenesis of pancreatic cancer is complicated and uncertain, there is still a lack of effective systemic treatment

Method used

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  • Application of SNP Markers in the Prognosis of Pancreatic Cancer
  • Application of SNP Markers in the Prognosis of Pancreatic Cancer
  • Application of SNP Markers in the Prognosis of Pancreatic Cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Screening of SNP sites associated with pancreatic cancer prognosis

[0046] 1. Research object

[0047] Blood samples from 44 patients with histologically confirmed locally advanced pancreatic cancer (IOERT-LAPC) treated with IOERT were collected by the National Cancer Center of China from January 2008 to November 2013, and all patients had no tumor metastasis.

[0048] 2. Extraction of blood genomic DNA

[0049] Use the blood DNA extraction kit SK8224 to extract the genomic DNA in the blood sample, and the specific operation is detailed in the instructions.

[0050] 3. Genotype detection

[0051] The extracted DNA was sequenced using Illumina HumanOmni5-4v1.

[0052] 4. Data Analysis

[0053] Use GenomeStudio to perform genotype calling and exclude samples with a genotype calling rate lower than 95%; perform correlation analysis on the data to exclude genotype calling rates lower than 95% and minimum allele frequency less than 0.05, Hardy-Weinberg SNPs w...

Embodiment 2

[0059] Example 2 Verifies the correlation between rs1026654 and IOERT-LAPC survival

[0060] 1. Research object

[0061] Blood samples from 83 patients with histologically confirmed locally advanced pancreatic cancer (IOERT-LAPC) treated by IOERT were collected by the National Cancer Center from December 2013 to April 2017. All patients had no tumor metastasis and no follow-up was excluded. There were 17 invalid samples of records, and 66 cases were finally included in the study.

[0062] 2. Extraction of blood genomic DNA

[0063] Use Shanghai Sangon's Rapid DNA Extraction Kit SK8224 to extract DNA from blood. For details, please refer to the operation manual.

[0064] 3. PCR amplification of the target gene

[0065] 1) Design primers

[0066] Design PCR primers according to the upstream and downstream sequences of rs1026654. The specific primer sequences are as follows:

[0067] Forward primer 5'-TGGTGGCAGAGTGAACTCATAGA-3' (SEQ ID NO.1),

[0068] Reverse primer 5'-ATAA...

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Abstract

The invention discloses an application of an SNP marker in pancreatic cancer prognosis. The rs1026654 is discovered to be related to the lifetime of an IOERT-LAPC patient for the first time by sequencing an IOERT-LAPC sample. Further, sanger sequencing is used for verification in the IOERT-LAPC patient. The invention finds that: when the genotype of the rs1026654 is AA, prognosis of the patient isgood; when the genotype of the rs1026654 is AG or GG, the prognosis is poor. The invention prompts that the prognosis of the IOERT-LAPC patient can be judged by detecting the genotype of the rs1026654.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of a SNP marker in the prognosis of pancreatic cancer, in particular to the SNP rs1026654. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract, which ranks thirteenth among common tumors and fourth among the leading causes of death due to tumors, and its mortality rate is almost equal to the incidence rate (Oberstein P E, Olive KP. Pancreatic cancer :why is it so hard to treat?[J].Therap Adv Gastroenterol,2013,6(4):321-337.). The high mortality rate of pancreatic cancer presents an obvious trend in developed countries. In 28 countries of the European Union, pancreatic cancer will surpass breast cancer and become the third leading cause of death. In recent years, as the population of developing countries has gradually adapted to the lifestyles of developed countries, such as smoking, high-calorie food, and less physical exerci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/156
Inventor 王成锋陈应泰孙跃民
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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