Laser device for treatment of wounds
A wound and laser technology, applied in the field of wound treatment, can solve problems such as low quality
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[0130] Objective: To confirm the relative amount of energy required to kill Escherichia coli (Escherichia coli, Escherichia coli) living cells and human fibroblast living cells respectively.
[0131] METHODS: Human fibroblasts and E. coli cells were stained with SYTO 9 dye and propidium iodide. Samples were prepared by mixing stained human fibroblasts, stained E. coli cells and low-melt agarose, which provided embedding of the cells. A drop is obtained from the sample and placed on a glass slide. Slides were placed under a conventional confocal microscope equipped with a 405 nm laser. Using standard confocal setups, UV ablation was used to selectively induce cell death and visualize single-cell responses in a dose-dependent manner. Viable cells are identified as green, while inactivated (dead) cells are identified as red. Identify living cells of E. coli and engage 405nm laser. The 405nm laser intensity was initially set low and then increased until one E. coli cell was ki...
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