Loading device, loading method and gene sequencing system

Abstract

The invention provides a loading device for loading different liquid reactants on a sequencing chip for biochemical reaction. The loading device comprises a cover plate and a base, a sealing groove and a runner groove are formed in the bottom surface of the cover plate; the runner groove is formed in the inner side of the sealing groove; the cover plate is propped against a sequencing chip througha sealing piece embedded into the sealing groove to form a cavity; the flow channel groove is provided with a liquid inlet and a liquid outlet, the liquid inlet or the liquid outlet crosses over thesealing groove through a corresponding flow channel in the cover plate and is communicated with an electromagnetic valve on the base, and the electromagnetic valve is used for controlling liquid adding and liquid discharging. Due to the application of the device, the sequencing chip does not need expensive packaging treatment, a reactant can be directly loaded on the bare sequencing chip, the costis low, the operation is convenient, no bubble is generated in the loading process, the liquid change is stable, and a rapid and large-flux automatic biological reaction is facilitated.

Description

technical field [0001] The invention relates to the field of gene technology, in particular to a loading device for loading several liquid reagents on a bare sequencing chip for biochemical reactions, a loading method, and a gene sequencing system using the device. Background technique [0002] This section is intended to provide a background or context for implementations of the invention that are recited in the claims. The descriptions herein are not admitted to be prior art by inclusion in this section. [0003] Gene sequencing chips (such as silicon wafers) need to undergo biological reactions with a variety of different reagents to be able to immobilize or carry gene molecules or nucleic acid molecules on their surfaces. During the loading of reagents, the speed, volume, time, and temperature of the liquid change , The stability of the liquid change needs to be strictly controlled, and the chip cannot be in contact with the air during the reagent replacement process, s...

AI Technical Summary

Problems solved by technology

[0004] The above silicon chip DNB loading method, the packaging process of each chip is complicated, and the accuracy and sealing of the flow channel are extremely high, and the scrap rate of the chip during packaging is high; at the same time, the demand for auxiliary materials (glass sheet, frame) is large and the cost is high. Not only that, each chip needs to be packaged, which is not conducive to flexible use, especially once the chip is filled with air bubbles, it is difficult to get rid of it, and it is not conducive to rapid and large-throughput automated biological reactions after packaging with glass sheets

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