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A cryoprotectant based on liquid metal nanoparticles and its method and application

A technology of cryoprotectant and liquid metal, which is applied in the field of biomedicine to achieve high light-to-heat conversion rate, uniform particle size distribution, and the effect of inhibiting the formation of ice crystals

Active Publication Date: 2021-10-01
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

During rewarming, the critical heating rate must exceed the critical cooling rate by at least an order of magnitude to prevent ice crystal formation in the cryoprotectant medium and system, and achieving faster warming rates at low concentrations of cryoprotectants remains challenging

Method used

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  • A cryoprotectant based on liquid metal nanoparticles and its method and application
  • A cryoprotectant based on liquid metal nanoparticles and its method and application
  • A cryoprotectant based on liquid metal nanoparticles and its method and application

Examples

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Embodiment 1

[0024] This embodiment provides a human bone marrow mesenchymal stem cell frozen balance liquid, cryoprotectant and thawing liquid. The components of the frozen balance liquid are: conventional culture medium and basic protective agent. The stem cell complete medium is composed of 60% fetal bovine serum, and the basic protective agents are ethylene glycol and propylene glycol, both of which have a concentration of 1M. The components of the cryoprotectant are: conventional culture medium and basic protective agent. The conventional culture medium is composed of 40% human bone marrow mesenchymal stem cell complete medium and 60% fetal bovine serum. The basic protective agents are ethylene glycol, propylene glycol and trehalose. The concentration of ethylene glycol and propylene glycol is 2M, and the concentration of trehalose is It is 0.5M. The thawing liquid component is PBS solution of 0.5M trehalose.

Embodiment 2

[0026] This embodiment provides a human bone marrow mesenchymal stem cell frozen balance liquid, cryoprotectant and thawing liquid. The components of the frozen balance liquid are: conventional culture medium and basic protective agent. The stem cell complete medium is composed of 60% fetal bovine serum, and the basic protective agents are ethylene glycol and propylene glycol, both of which have a concentration of 1M. The components of the cryoprotectant are: liquid metal nanoparticles, conventional culture fluid and basic protective agent. The liquid metal nanoparticles are GaIn 24.5 The concentration of the alloy (average particle size is 100-200nm) is 0.1 mg / mL, the conventional culture medium is composed of 40% human bone marrow mesenchymal stem cell complete medium and 60% fetal bovine serum, and the basic protective agents are ethylene glycol, propylene glycol and For trehalose, the concentration of ethylene glycol and propylene glycol is 2M, and the concentration of tr...

Embodiment 3

[0028] This embodiment provides a cryoprotectant for human bone marrow mesenchymal stem cells. The preparation method is the same as that in Embodiment 2, except that the concentration of nano liquid metal is 0.2 mg / mL.

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Abstract

The invention relates to a cryoprotectant based on liquid metal nanoparticles and its method and application, including liquid metal nanoparticles and a basic protective agent; the melting point of the raw material of the liquid metal nanoparticles is below 60°C, and the basic protective agent is Ethylene Glycol, Propylene Glycol and Trehalose. The cryoprotectant can realize vitrification in the process of cooling down, and in the process of rewarming, it can be used as a space heat source under the irradiation of near-infrared laser, which can greatly increase the heating rate, inhibit devitrification, reduce the formation of ice crystals, and improve the efficiency of frozen storage. Cellular and vascular tissue viability and structural integrity.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a cryoprotectant based on liquid metal nanoparticles and its method and application. Background technique [0002] Cryopreservation is the only method for long-term preservation of valuable cells to maintain the functional properties of cells (such as immunomodulatory capacity, differentiation capacity) and to reach the quantity required for clinical application. Recently, various methods for cryopreservation have been developed, including slow freezing and vitrification. Slow freezing methods are time consuming and often require expensive programmed cooling equipment to achieve the desired cooling rate. Compared with slow freezing, since vitrification is a freezing mode that rapidly lowers the cell temperature, it avoids mechanical damage to cells during slow freezing, shortens the freezing process time, simplifies steps, and does not require Expensive programmed cooling equipment, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02B22F1/00B22F9/06
CPCA01N1/0221B22F9/06B22F1/054
Inventor 后仪饶伟刘静
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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