Tumor cell content evaluation method, device and system and computer readable medium
A tumor cell and evaluation device technology, applied in the directions of proteomics, instrumentation, genomics, etc., can solve problems such as difficulties, large differences in results, and large human errors in pathological staining, and achieve a high degree of proximity effect.
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Embodiment 1
[0056] This example is to illustrate the tumor cell content evaluation system and the corresponding processing process.
[0057] figure 2 A structural block diagram of the tumor cell content evaluation system involved in Example 1 of the present invention.
[0058] Such as figure 2 As shown, the tumor cell content evaluation system 100 includes: a mutation frequency detection device 10 , and a tumor cell content evaluation device 20 communicatively connected to the mutation frequency detection device 10 through a communication network 30 .
[0059] The mutation frequency detection device 10 is based on the results of sequencing the detection sample and the control sample, and analyzes it to obtain a sequencing sequence comprising each average sample mutation frequency corresponding to each mutation site and a control mutation frequency corresponding to each mutation site. Analyze the data, that is, obtain the mutation frequency of each mutation site in the detection sample...
Embodiment 2
[0096] The following is a description of Embodiment 2.
[0097] In Example 2, the same symbols are assigned to the same structures as in Example 1, and the same descriptions are omitted.
[0098] Figure 5 It is a structural block diagram of the tumor cell content assessment device involved in Example 2 of the present invention.
[0099] Such as Figure 5 As shown, the tumor cell content evaluation device 220 has a single tumor cell content calculation unit 21, a tumor content calculation unit 22, a content evaluation unit 223, an information storage unit 24, a tumor cell content evaluation side communication unit 25, and a tumor cell content evaluation side temporary storage unit. Part 26 and tumor cell content evaluation side control part 27.
[0100] In this embodiment, the difference between the tumor cell content evaluation device 220 and the embodiment is the predetermined evaluation rule adopted by the content evaluation unit 223. The predetermined evaluation rule in...
Embodiment 3
[0106] This example is to verify the reliability of the tumor cell content assessment device in the tumor cell content assessment system in Example 1 and the corresponding processing process for evaluating the tumor cell content in samples. details as follows.
[0107] Sample selection: Select paired tumor cell line samples and normal cell line samples. The purchased commercial cell lines are shown in Table 2. The tumor cell lines are all pure tumor cells, that is, 100% tumor cells, and the normal cell lines are all pure tumor cells. normal cells, that is, 100% normal cells.
[0108]
[0109] Mixing is done in two ways:
[0110] Method 1: Before sequencing (experimental stage), DNA extracted from tumor cell lines and DNA extracted from normal cell lines were mixed according to different ratios. Targeted sequencing.
[0111]
[0112] Method 2: In the data analysis stage, perform data extraction and mixed samples, specifically: first sequence the 5 pure cell lines in Ta...
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