PCR centrifugal microfluidic device and method

Abstract

The invention relates to a polymerase chain reaction (PCR) centrifugal microfluidic device. The device comprises a chip and thermostatic blocks; the chip is provided with at least two reaction chambers for holding reagents, the thermostatic blocks are different in temperature and arranged on the chip at intervals, and the thermostatic blocks are one-to-one corresponding to the reaction chambers and fixed relative to the reaction chambers; and the reagents can flow among different reaction chambers for periodical switching, and the different thermostatic blocks alternately heat the reagents. The manner of replacing traditional time temperature cycle with the spatial temperature cycle can reduce time required for one cycle of a PCR reaction of the reagents and improve the reaction efficiency; and at the same time, a traditional PCR reaction variable-temperature control mode is replaced with a constant-temperature control mode, so that the technical difficulty of temperature rise and fallcontrol is greatly reduced, the thermostatic blocks improve the accuracy of temperature required by the reagents in different reaction stages, and the PCR reaction effect of the reagents is ensured.

Description

technical field [0001] The invention relates to the technical field of polymerase chain reaction, in particular to a PCR centrifugal microfluidic device and a method thereof. Background technique [0002] Polymerase chain reaction is referred to as PCR (Polymerase Chain Reaction). PCR is a method for enzymatically synthesizing specific DNA fragments in vitro. Periodic cycle treatment is carried out through steps such as heating at different temperatures, and then the target DNA can be rapidly amplified. PCR has the characteristics of strong specificity, high sensitivity, easy operation, and time-saving; it can not only be used for basic research such as gene isolation, cloning, and nucleic acid sequence analysis, but also for disease diagnosis. [0003] Generally, the traditional microfluidic device heats the chip through the air to realize the PCR reaction. On the one hand, due to the uneven heating of the air, the temperature in different regions cannot be reached quickly...

AI Technical Summary

Problems solved by technology

[0003] Generally, the traditional microfluidic device heats the chip through the air to realize the PCR reaction. On the one hand, due to the uneven heating of the air, the temperature in different regions cannot be reached quickly, making it difficult to ensure temperature control.

On the other hand, a large heating space cannot achieve rapid temperature rise and fall, making the PCR reaction time longer

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