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Bifidobacterium breve and application thereof in preparation of conjugated fatty acid

A technology of bifidobacterium breve and conjugated linolenic acid, applied in the field of microorganisms, can solve the problems of inability

Active Publication Date: 2020-03-13
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the wide variety of Bifidobacterium genus, there are significant differences in morphology, physiology, metabolism and physiological functions between Bifidobacteria of the same genus and different species. So far, a small number of studies have found that some Bifidobacteria breve can produce conjugated linoleum Acid, conjugated linolenic acid, some can not, and there is no research to determine the cause or mechanism of the difference

Method used

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  • Bifidobacterium breve and application thereof in preparation of conjugated fatty acid
  • Bifidobacterium breve and application thereof in preparation of conjugated fatty acid
  • Bifidobacterium breve and application thereof in preparation of conjugated fatty acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Collection of samples and isolation and identification of bifidobacteria

[0036] (1) Collection of samples and isolation of bifidobacteria

[0037] Feces samples from infants in Changping District, Beijing were collected at Xingchang Jiayuan, Nanshao Town, Changping District, Beijing.

[0038] Take 1g of feces sample, spread it on mMRS solid medium after serial dilution, culture it at 37°C in an anaerobic environment for 72 hours, observe and record the colony shape, pick the colony and purify it, and then put it in mMRS liquid medium at 37°C Cultivate at ℃ for 48 hours, perform Gram staining on the obtained colonies and record the strain morphology, discard the Gram-negative strains and Gram-positive cocci in the colonies, and select the Gram-positive bacilli, which are discarded after catalase analysis. In addition to the catalase-positive strains, the catalase-negative strains were retained, and the negative strains were discarded by the detection of fru...

Embodiment 2

[0053] Example 2: Application of Bifidobacterium breve FBJCP2M1 in the preparation of conjugated linoleic acid

[0054] 1. Strain activation

[0055] Take out the glycerol tube containing Bifidobacterium breve FBJCP2M1 from the -80°C refrigerator, take the bacterial solution and streak it on the mMRS solid medium, and culture it at 37°C for 48h in an anaerobic environment. The grown single colonies were picked and inoculated in mMRS liquid medium, cultured at 37°C for 48 hours under anaerobic environment, and continuously activated for 3 generations.

[0056] 2. Preparation of linoleic acid mother liquor

[0057] Weigh 300mg of linoleic acid (LA) and 200mg of Tween-80, dissolve in water and dilute to 10mL, stir and emulsify thoroughly, filter and sterilize through a 0.45μm sterile filter membrane, and store at -20°C in the dark.

[0058] 3. Co-culture with linoleic acid

[0059] Inoculate the activated bacterial solution in step 1 into 10 mL of mMRS liquid medium containing...

Embodiment 3

[0072] Example 3: Application of Bifidobacterium breve FBJCP2M1 in the preparation of conjugated linolenic acid

[0073] 1. Strain activation

[0074] Take out the glycerol tube containing Bifidobacterium breve FBJCP2M1 from the -80°C refrigerator, take the bacterial solution and streak it on the mMRS solid medium, and culture it at 37°C for 48h in an anaerobic environment. The grown single colonies were picked and inoculated in mMRS liquid medium, cultured at 37°C for 48 hours under anaerobic environment, and continuously activated for 3 generations.

[0075] 2. Preparation of linolenic acid mother liquor

[0076] Weigh 300mg of α-linolenic acid (α-LNA) and 200mg of Tween-80, dissolve in water and make up to 10mL, stir and emulsify thoroughly, filter and sterilize through a 0.45μm sterile filter membrane, and store at -20°C, protected from light .

[0077] 3. Co-culture with linolenic acid

[0078]Inoculate the bacterium solution activated in step 1 into 10 mL mMRS liquid...

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Abstract

The invention relates to bifidobacterium breve and an application thereof in preparation of conjugated fatty acid, belonging to the technical field of microorganisms. The invention provides bifidobacterium breve FBJCP2M1 capable of producing conjugated linoleic acid, conjugated linolenic acid and conjugated octadecatetraenoic acid, wherein the conversion rate of linoleic acid with the bifidobacterium breve FBJCP2M1 to produce the conjugated linoleic acid can reach 87.98%, and the content of the c9,t11-CLA isomer accounts for 98.38% of the content of the conjugated linoleic acid; the conversionrate of linolenic acid with the bifidobacterium breve FBJCP2M1 to produce the conjugated linolenic acid can reach 95.6%, and the content of the c9,t11,c15-CLNA isomer accounts for 95.01% of the content of the conjugated linolenic acid; and the conversion rate of octadecatetraenoic acid with the bifidobacterium breve FBJCP2M1 to produce the conjugated octadecatetraenoic acid can reach 55.86%, andthe content of c6,c9,t11,c15-CSA isomer accounts for 90.45% of the content of the conjugated octadecatetraenoic acid.

Description

technical field [0001] The invention relates to a strain of bifidobacterium breve and its application in preparing conjugated fatty acids, belonging to the technical field of microorganisms. Background technique [0002] Conjugated linoleic acid (CLA) is the general term for octadecadienoic acid containing conjugated double bonds, and it is the positional isomer and geometric isomer of linoleic acid (Linoleic acid, 18:2) . The most common isomer is cis 9, trans 11-CLA (c9, t11-CLA), also known as rumenic acid. In addition, trans 10, cis 12-CLA (t10, c12-CLA) is also an isomer with relatively high content in nature. Conjugated linoleic acid has attracted attention because of its biological functions. Different conjugated linoleic acid isomers have different physiological functions, among which c9,t11-CLA and t10,c12-CLA are recognized as the most physiologically active co- Conjugated linoleic acid isomers, c9, t11-CLA, mainly function in anti-cancer, anti-inflammation and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/64C12R1/01
CPCC12N1/20C12P7/6427C12N1/205C12R2001/01
Inventor 陈卫杨波陆文伟王顺余何建新郑宋友李国平付成丽朱玲丽赵建新张灏
Owner JIANGNAN UNIV
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