Material and material box for separation, culture and storage of dental pulp mesenchymal stem cells

A mesenchymal stem cell and cell culture technology, applied in the field of stem cell culture, can solve the problems of multiple types of materials and tools, the inability to study or utilize dental pulp mesenchymal stem cells, and long time consumption

Pending Publication Date: 2020-03-27
深圳市润科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of obtaining dental pulp mesenchymal stem cells, there are many types of materials and tools used, and the individual configuration takes a long time, and it is easy to introduce the risk of contamination
In addition, the materials used must ensure that the viability, cell number, adipogenic induction, osteogenic induction and expression of surface markers of dental pulp mesenchymal stem cells are not affected after cryopreservation, otherwise, the obtained dental pulp mesenchymal stem cells cannot be studied or exploited

Method used

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  • Material and material box for separation, culture and storage of dental pulp mesenchymal stem cells
  • Material and material box for separation, culture and storage of dental pulp mesenchymal stem cells
  • Material and material box for separation, culture and storage of dental pulp mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 30ml tooth preservation solution, The second bottle of 10ml tooth cleaning solution, the third bottle containing 10ml tooth disinfection solution and the fourth bottle containing 10ml tooth cleaning solution; the cell culture set consists of 500ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 5ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 5ml PALL TM The seventh bottle of Ultroser G, containing 5ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 500ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 50ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 50ml of the first cryopreservat...

Embodiment 2

[0046] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 20ml tooth preservation solution, The second bottle of 5ml tooth cleaning solution, the third bottle containing 5ml tooth disinfection solution and the fourth bottle containing 5ml tooth cleaning solution; the cell culture set consists of 400ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 4ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 4mlPALL TM The seventh bottle of Ultroser G, containing 4ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 400ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 40ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 40ml of the first cryopreservation ...

Embodiment 3

[0050] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 40ml tooth preservation solution, The second bottle of 15ml tooth cleaning solution, the third bottle containing 15ml tooth disinfection solution and the fourth bottle containing 15ml tooth cleaning solution; the cell culture set consists of 600ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 6ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 6mlPALL TM The seventh bottle of Ultroser G, containing 6ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 600ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 60ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 70ml of the first cell cryoprese...

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Abstract

The invention provides a material and material box for separation, culture and storage of dental pulp mesenchymal stem cells. The material is composed of a dental pulp collection liquid, a cell culture medium, a cell culture auxiliary liquid, and a cell cryopreservation liquid, wherein the dental pulp collection liquid is composed of a tooth preservation liquid, a tooth cleaning liquid and a toothdisinfection liquid; the cell culture medium is composed of four reagents after mixing; the cell culture auxiliary liquid is composed of two reagents after mixing; and the cell cryopreservation liquid is composed of a first cell cryopreservation liquid and a second cell cryopreservation liquid, the first cell cryopreservation liquid is composed of MSCGM-CDTM BulletKit and Ultroser G according toa weight ratio of (1-10):1, and the second cell cryopreservation liquid is composed of DMSO and dextran according to a weight ratio of DMSO to dextran = 1:(0-2). The material does not affect viability, cell number, adipogenic induction, osteogenic induction and expression of surface markers of the dental pulp mesenchymal stem cells.

Description

Technical field [0001] The present invention relates to the technical field of stem cell culture, in particular to a material and a material box for separating, culturing and storing dental pulp mesenchymal stem cells. Background technique [0002] Stem cells have the potential to regenerate various tissues and organs and the human body, so they are called "universal cells" by the medical community. The human body is composed of more than 200 types of cells with a total of about 50-75 trillion cells. About 300 million cells die every minute. At the same time, a considerable number of new cells are produced to form metabolism. These new cells are all stem cells. In recent years, with the deepening of research, it has been possible to extract and cultivate various stem cells from umbilical cord blood, umbilical cord, placenta and other tissues. The number of stem cells in dental pulp is equivalent to a human body's stem cell backup, which is a precious life resource. [0003] Compar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCC12N5/0664A01N1/0221C12N2509/00
Inventor 魏宗科林科佳马冬磊张若楠
Owner 深圳市润科生物科技有限公司
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