Material and material box for separation, culture and storage of dental pulp mesenchymal stem cells
A mesenchymal stem cell and cell culture technology, applied in the field of stem cell culture, can solve the problems of multiple types of materials and tools, the inability to study or utilize dental pulp mesenchymal stem cells, and long time consumption
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Embodiment 1
[0042] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 30ml tooth preservation solution, The second bottle of 10ml tooth cleaning solution, the third bottle containing 10ml tooth disinfection solution and the fourth bottle containing 10ml tooth cleaning solution; the cell culture set consists of 500ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 5ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 5ml PALL TM The seventh bottle of Ultroser G, containing 5ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 500ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 50ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 50ml of the first cryopreservat...
Embodiment 2
[0046] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 20ml tooth preservation solution, The second bottle of 5ml tooth cleaning solution, the third bottle containing 5ml tooth disinfection solution and the fourth bottle containing 5ml tooth cleaning solution; the cell culture set consists of 400ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 4ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 4mlPALL TM The seventh bottle of Ultroser G, containing 4ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 400ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 40ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 40ml of the first cryopreservation ...
Embodiment 3
[0050] The material box used for the separation, culture and storage of dental pulp mesenchymal stem cells includes a dental pulp collection kit, a cell culture kit and a cell cryopreservation kit; the dental pulp collection kit includes a first bottle containing 40ml tooth preservation solution, The second bottle of 15ml tooth cleaning solution, the third bottle containing 15ml tooth disinfection solution and the fourth bottle containing 15ml tooth cleaning solution; the cell culture set consists of 600ml MSCGM-CD TM The fifth bottle of Bullet Kit, containing 6ml Gibco TM The sixth bottle of GlutaMAXSupplement, containing 6mlPALL TM The seventh bottle of Ultroser G, containing 6ml Gibco TM The eighth bottle of Penicillin-Streptomycin-Glutamine, containing 600ml Gibco TM The ninth bottle of 0.25% Trypsin and contains 60ml Gibco TM The tenth bottle of DPBS is composed; the cell cryopreservation kit is composed of the eleventh bottle containing 70ml of the first cell cryoprese...
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