Linked KASP molecular markers for high temperature tolerance traits of rice and applications thereof

A technology of molecular markers and high temperature resistance, which is applied in the field of linked KASP molecular markers for high temperature resistance traits of rice, can solve the problems of high cost, achieve low cost, improve the efficiency of variety breeding, and promote the effect of genetic improvement

Inactive Publication Date: 2020-03-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

DNA chip technology is the most common method to detect SNP, but the cost of this method is high

Method used

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  • Linked KASP molecular markers for high temperature tolerance traits of rice and applications thereof
  • Linked KASP molecular markers for high temperature tolerance traits of rice and applications thereof
  • Linked KASP molecular markers for high temperature tolerance traits of rice and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0048] The primer development and preparation method of molecular markers 7238, 6400, 7334, the steps are:

[0049] According to the inventor's previous research, a new set of chromosome segment substitution lines (CSSLs) was established using the high temperature tolerant indica rice variety N22 and the high temperature sensitive indica rice variety 9311, and their genetic basis was analyzed. The effects of high temperature stress on rice seedling and heading stages were studied using relative electrical conductivity (REC) and seed setting rate (SSR). This study clarified that the high temperature tolerance of rice at the heading stage has a significant positive correlation with its seed setting rate under high temperature stress. And the study identified five QTLs for high temperature resistance: qSSR6-1, qSSR7-1, qSSR8-1, qSSR9-1 and qSSR11-1. Among them, qSSR7-1 and qSSR11-1 explained higher phenotypic variation, which were 26.35% and 14.21%, respectively. For the two QT...

Embodiment 2

[0068] The primer application method of KASP molecular marker 7238,6400,7334, its steps are:

[0069] (1) Using the KASP molecular markers 7238, 6400, 7334 and genotyping methods provided by the summary of the invention, 10 individual plants of the 9311 / N22 CSSLs population were genotyped, and QTLqSSR7-1 and QTLqSSR11 with high temperature resistance were screened out Lines CSSL74, CSSL105 of -1 genome segment. CSSL74 carried the high temperature resistant QTLqSSR7-1, and CSSL105 carried the high temperature resistant QTLqSSR11-1.

[0070] (2) The two individual plants obtained in step (1) are crossed to obtain F 1 Seeds, planted in the field to get F 1 Generation single plant, each F 1 F 2 generation seeds.

[0071] (3) The F obtained in step (2) 2 Generation seeds were germinated in the laboratory, DNA was extracted, and 1200 strains of F 2 Genotype identification was carried out on the seedlings, and 52 homozygous individual plants that aggregated QTL qSSR7-1 and QTL...

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Abstract

The invention discloses a set of linked KASP molecular markers for the high temperature tolerance traits of rice and applications thereof. The molecular markers are 7238, 6400 and 7334; the molecularmarker 7238 is the linked marker of QTL interval qSSR7-1, and the molecular marker 6400 and 7334 are the linked markers of QTL interval qSSR11-1; and the molecular marker 7238, 6400 and 7334 respectively are the variation C / T at the 29287238 base of chromosome 7, the variation A / C at the 26796400 base of chromosome 11 and the variation A / G at the 26797334 base of the chromosome 11. The molecular markers or linked KASP molecular marker primers can be applied to the improved breeding for the high temperature tolerance of rice.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a linked KASP molecular marker for high temperature resistance traits of rice and its application. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the population uses rice as a staple food. High temperature is the main stress in rice production, and it can cause 50% to 80% reduction in rice yield in severe cases. Breeding and planting high temperature resistant varieties is an effective method to ensure the safety of rice grain production. Although great progress has been made in the study of high temperature tolerance in rice, the high temperature tolerance traits are mainly quantitative traits, and the genetic basis of high temperature tolerance traits in rice seedling and heading stages is still unclear. The traditional breeding method relying on high-temperature screening to identify resistant varieties an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 陈庆全胡群文程梦瑶王璐杨
Owner ANHUI AGRICULTURAL UNIVERSITY
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