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Method for removing nucleus of oocyte through chemical induction

A chemical induction and egg cell technology, which is applied in the field of chemical induction to remove the nucleus of egg cells, can solve the problems of high operator skills, large mechanical damage to cells, and low damage tolerance, and is suitable for popularization and application. Small amount of effect

Inactive Publication Date: 2020-03-31
WUHU INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Egg cells tend to have low tolerance to damage during mechanical enucleation, affecting enucleation efficiency
The disadvantage of this method is that the mechanical damage to the cells is relatively large, the equipment requirements are high, the operator is highly skilled and time-consuming

Method used

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  • Method for removing nucleus of oocyte through chemical induction
  • Method for removing nucleus of oocyte through chemical induction
  • Method for removing nucleus of oocyte through chemical induction

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Embodiment Construction

[0017] For the following, through the description of the examples, the specific implementation of the present invention, such as the manufacturing process involved, the operation and use method, etc., will be described in further detail, so as to help those skilled in the art to have a more complete concept of the invention and the technical solution of the present invention. , accurate and in-depth understanding.

[0018] It should be noted that all the expressions of "once" and "twice" in the embodiments of the present invention are used to distinguish two entities with the same name but different parameters or different parameters. It can be seen that "once" and "twice" are only for The convenience of expression should not be interpreted as a limitation on the embodiments of the present invention, which will not be described one by one in the subsequent embodiments. In the examples of the present invention, GV is the germinal vesicle period, and GVBD is the germinal vesicle...

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Abstract

The invention discloses a method for removing the nucleus of an oocyte through chemical induction. The in-vitro oocyte is induced and treated by decarboxylated colchicine and cycloheximide, and in thefirst division later period of the oocyte after a certain period of time, chromosomes cannot be separated and discharged along with a polar body. The method for removing the nucleus of the oocyte through chemical induction is easy and convenient to operate, a large number of nucleus-free oocytes can be obtained, the nucleus removing efficiency is high, and the method is suitable for application and popularization.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for chemically inducing the removal of egg cell nuclei. Background technique [0002] Since the beginning of the 21st century, great achievements have been made in the application of life sciences, especially animal cloning technology to genetic breeding, and have made great contributions to human agriculture and life and health. Somatic cell nuclear transfer cloning technology has successively cloned pigs, cattle, sheep, mice and other animals in mammals. It provides a new research method for life science, human medicine and many other fields. The technology of somatic cell nuclear transfer involves several key technologies, among which the removal of oocyte nuclear material is one of the key technologies. [0003] The number of removed oocyte nuclei is an important factor related to the construction of reconstructed embryos. In the current situation of low nucle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075C12N15/01
CPCC12N5/0609C12N15/01C12N2500/02C12N2501/999
Inventor 王巨龙黎婷张海燕刘宽辉戴菁晶邹文瑞
Owner WUHU INST OF TECH
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