An automatic pressing cellulose acetate film spotter

Abstract

The invention relates to an automatic pressing cellulose acetate film spotting device, which includes a spotting plate, a bracket, a casing, and a driving casing. A driving screw is movably connected in the driving casing, and a driving motor is fixedly arranged on the top of the driving casing. The lead screw is threadedly connected with a lead screw sleeve; the drive housing on the other side of the shell is fixedly connected with a guide rod, and the guide rod is sleeved with a guide cylinder that moves up and down along the guide rod; the lower part of the shell is provided with a connecting plate for connecting There is a drive cross bar above the plate, and the two ends of the drive cross bar pass through the vertical holes on both sides of the shell to be disassembled and connected with the screw sleeve and the guide cylinder respectively; the connecting plate and the dot template are connected by multiple damping springs ;Avoid the situation that the film is split or the lines are not obvious caused by excessive or too small force, and the success rate of cellulose acetate film spotting film is guaranteed.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to an automatic pressing cellulose acetate film spotter. Background technique [0002] Electrophoresis is an experimental technique commonly used in biochemical experiments. It is often used for qualitative and quantitative analysis of DNA and proteins. An electrophoresis apparatus that uses a thin film made of cellulose acetate as a support is called thin film electrophoresis. Under certain pH conditions, protein molecules have a certain charge due to group ionization, and move in a certain direction at a certain speed under the action of an electric field force on a film containing a buffer. Different protein molecules move at different speeds and directions due to their different charges, thereby achieving separation. This kind of film has little adsorption effect on protein samples, and almost completely eliminates the tailing phenomenon in paper protein electrophoresis, ...

AI Technical Summary

Problems solved by technology

[0003] The existing cellulose acetate film electrophoresis spotting device is on both sides of the spotting plate used for dipping samples, and there is a liquid removal plate with the same width but a small height, which is connected to the support arm through a spring to ensure Press the anti-skid groove to push the liquid removal plate downward to dip the solution, the liquid removal plates on both sides will be close to the middle point sample, and the three plates are in contact with the sample solution at the same time, so that most of the solution is squeezed out of the entire device, thereby avoiding the middle point There is a lot of solution accumulated on both sides of the sample; in addition, when the finger is released after sampling, the liquid removal plates on both sides will shrink due to the tension of the spring, and at the same time, because the upper end of the support arm is designed with a handle, you can hold the handle with your hand and point on the acetate film. so as to ensure that a thick line can be made. This spotter is simple in structure, novel and unique, easy to operate, good in spotting effect, greatly improves work efficiency, economical and practical, but also has disadvantages, that is, the intensity of spotting is controlled by humans. Yes, and this force needs to be mastered by experience, it is not easy to control, but the cellulose acetate film is very thin, if the force is too large, the film will be easily broken; great inconvenience

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