Germplasm improving method for asparagus beans

A technology of cowpea and germplasm, applied in the fields of plant genetic improvement, botanical equipment and methods, applications, etc., can solve the problems of weak research foundation of distant hybridization, improvement of insect resistance of long cowpea, etc.

Pending Publication Date: 2020-04-10
JIANGHAN UNIVERSITY
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Problems solved by technology

However, the research basis of corresponding distant hybridization in the edible beans of the genus Vigna is relatively weak, and there is no successful case of using distant hybridization to improve the insect resistance of long Vigna

Method used

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  • Germplasm improving method for asparagus beans

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Embodiment

[0031] Distant hybridization to construct segregated populations:

[0032] Long cowpea A147 was selected as the female parent, with light green pods, white corolla, long pods, and early-medium maturity; rice bean A111 was selected as the male parent, with dark green pods, purple-red corolla, short pods, and mid-to-late maturity. From 2012 to 2014, through several times of distant hybridization, in June 2014, a distant hybrid combination of long cowpea and rice bean was obtained (cowpea A147 was the female parent and rice bean A111 was the male parent) F 1 4 generation seeds; put F 1 All generation seeds were sown to obtain 867 F 2 Subsequent generation seeds, from 2015 to 2017, continuously add generation to F 6 generation. Specific process: F 2 Generational single-seed seeding, obtained the full separation spectrum of F 3 generation; respectively sow F 3 Generation seeds, randomly select a representative individual plant in its strain population, and obtain F 4 substit...

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Abstract

The invention provides a germplasm improving method for asparagus beans. The germplasm improving method for asparagus beans comprises the following steps of selecting rice beans with strong insect resistance and asparagus bean parents with weak insect resistance for distant hybridization to obtain F1 generation grains; sowing the F1 generation grains to obtain F2 generation seeds; sowing single F2generation grains to obtain a full split-spectrum F3 generation; separately sowing F3 generation seeds and randomly selecting representative single plants in a plant line group to obtain F4 generation seeds; then repeating the above steps to add generations to an F5 generation and an F6 generation; planting a full split-spectrum F6 generation plant line separated by distant hybridization in a field, and selecting distant hybridization recombinant single plants which are relatively strong in insect resistance and good in pod character from the full split-spectrum F6 generation plant line preliminarily to form an F7 generation; and sowing the F7 generation to obtain a plant line with strong insect resistance, and agronomic traits and quality character close to those of the asparagus bean parents. According to the germplasm improving method, the insect resistance of the asparagus beans is improved successfully by means of distant hybridization.

Description

technical field [0001] The invention belongs to the field of plant germplasm innovation, and in particular relates to a method for improving long cowpea germplasm. Background technique [0002] Cowpea (Vigna unguiculata L.) is a species of Fabaceae, Papilionaceae, and genus Vigna. China is the secondary birthplace of long cowpea and has a long history of planting. Because of its rich nutrition, it is widely loved by consumers. According to incomplete statistics, in 2017, the planting area of ​​long cowpea was as high as 330,000 hm 2 However, it is vulnerable to pests and diseases during its growth period, which often leads to a large amount of pesticides being released in the field, causing food quality problems and environmental problems. According to the survey, the main diseases in the field are gray mold, powdery mildew, rust, etc., and the main pests are Spodoptera litura, Beet Spodoptera, Bean Pod Borer, Thrips, etc. Spodoptera litura and beet armyworm are more har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 陈禅友潘磊郭瑞万何平邵景杰
Owner JIANGHAN UNIVERSITY
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