Microbial composite water purifying agent and preparation method thereof

A compound water purifying agent and microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unsatisfactory sewage treatment, unclear source of bacteria, limited use conditions, etc., and achieve recovery Water self-purification ability, rapid reproduction, and low cost effects

Pending Publication Date: 2020-04-10
广东慧信环保有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, microbial water purifiers, as a class of efficient and safe water purifiers, have been valued and recognized by many researchers, but the existing microbial wate

Method used

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  • Microbial composite water purifying agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A preparation method for a composite microbial preparation, comprising the following steps:

[0059] (1) Collect river and lake water samples and sediment, centrifuge, inoculate the supernatant in NB nutrient broth medium, and culture at 26°C for 48 hours to obtain water body bacterial liquid;

[0060] (2) Take the water body bacterial liquid for gradient dilution, and then spread it on the NB nutrient broth solid medium, culture it at 26°C for 12-72h, and isolate a single colony. Identification screening.

[0061] (3) The screened Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus acidophilus, Bacillus coagulans, Bacillus licheniformis and Pseudomonas aeruginosa were respectively expanded and cultured, and then the above-mentioned strains were mixed according to the ratio of viable bacteria , to obtain a composite microbial preparation.

[0062] The composite microbial preparation is composed of Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus a...

Embodiment 2

[0073] A preparation method for a composite microbial preparation, comprising the following steps:

[0074] (1) Collect water samples and sediment from rivers and lakes, centrifuge, inoculate the supernatant in NB nutrient broth medium, and culture at 27°C for 60 hours to obtain water body bacterial liquid;

[0075] (2) Take the water body bacterial liquid for gradient dilution, and then spread it on the NB nutrient broth solid medium, culture it at 27°C for 12-72h, and isolate a single colony. Identification screening.

[0076] (3) The screened Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus acidophilus, Bacillus coagulans, Bacillus licheniformis and Pseudomonas aeruginosa were respectively expanded and cultured, and then the above-mentioned strains were mixed according to the ratio of viable bacteria , to obtain a composite microbial preparation. The composite microbial preparation is composed of Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus aci...

Embodiment 3

[0090] The microbial composite water purifying agent prepared in Example 1-2 and the commercially available water purifying agent of the comparative example were subjected to sewage treatment test.

[0091] Specific experimental scheme: the microbial composite water purifier prepared in Example 1-2 and the commercially available water purifier of the comparative example were added to the sewage treatment pool at 0.3g / L, and the container prepared by Guangdong Huixin Environmental Protection Co., Ltd. was used at the same time The formula A-H composite river and lake water purifier increases oxygen in the water body, and detects the untreated river water in the sewage treatment tank and the purified water after 8 hours of treatment. The results of various indicators are shown in Table 1.

[0092] Table 1 Sewage Treatment Test Results

[0093]

[0094] Note: Transparency was measured by Sebster disc method; dissolved oxygen was measured by iodometric method; oxidation-reducti...

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Abstract

The invention relates to a microbial composite water purifying agent. The microbial composite water purifying agent is composed of a composite microbial preparation and a water purifying agent composite carrier, wherein the composite microbial preparation comprises bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa; the water purifying agent composite carrier comprises polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell charcoal, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite. The microbial composite water purifying agent adopts indigenous microorganisms in river and lake water bodies and bottom mud, and is rich in resource, easyto obtain and low in cost; the indigenous microorganisms subjected to enlarged culture are rapidly propagated and can be cooperated with the water purifying agent to degrade a large amount of pollutants in water, so that a carbon source is converted into carbon dioxide, a nitrogen source is converted into nitrogen, the pollutant concentration is greatly reduced, and the water body becomes clear.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and relates to a microbial composite water purifying agent and a preparation method thereof. Background technique [0002] At present, it is difficult to avoid the existence of acrylamide in synthetic organic polymer water purifiers. Acrylamide itself is a highly toxic substance, which is harmful to human health. Today, when people are desperately looking for a new type of water purifier to replace the traditional water purifier, microbial water purifiers have emerged as the times require. Microbial water purifiers have many unique properties and advantages: degradable, non-toxic and harmless, high safety, No secondary pollution. Therefore, microbial water purifiers, as a class of efficient and safe water purifiers, have been valued and recognized by many researchers, but the existing microbial water purifiers still have the following defects: large dosage, unstable effect, unclear sour...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12R1/125C12R1/07C12R1/23C12R1/10C12R1/385
CPCC02F3/34C12N1/20
Inventor 谭铭卓
Owner 广东慧信环保有限公司
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