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Primer group for detecting genotypes of oxytocin receptor and application thereof

An oxytocin receptor and primer set technology, which is used in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc.

Inactive Publication Date: 2020-04-10
北京德能安康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] To this end, the embodiment of the present invention provides a primer set for detecting the oxytocin receptor genotype and its application to solve the problem that there is no product in the prior art to guide clinical vaginal delivery and cesarean delivery from the perspective of gene expression differences crowd problem

Method used

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  • Primer group for detecting genotypes of oxytocin receptor and application thereof
  • Primer group for detecting genotypes of oxytocin receptor and application thereof
  • Primer group for detecting genotypes of oxytocin receptor and application thereof

Examples

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Embodiment 1

[0028] Example 1. Preparation of primer set and kit for detecting oxytocin receptor SNP genotype

[0029] The oxytocin receptor gene OXTR of the embodiment of the present invention is located on 3p25-3p26, includes 4 exons and 3 introns, and has a length of 19,206 base pairs. rs53576[Homo sapiens], Variant type SNV, Alleles: A>G, T, Chromosome: 3:8762685, Homo sapiens chromosome 3, GRCh38.p13 Primary Assembly. The nucleotide sequence of the rs53576 site polymorphism genome is shown in SEQ ID NO:5.

[0030] The primer probe sequences designed for the rs53576 SNP are as follows:

[0031] SEQ ID NO: 1, Rs53576-F: CACACCTCGGGCACAGCA;

[0032] SEQ ID NO: 2, Rs53576-R: CATCTGTAGAATGAGCTTCCCAGC;

[0033] SEQ ID NO: 3, first probe 76FAm: FAM-CCCGAGGATCCTC-MGB

[0034] SEQ ID NO: 4, second probe 76VGm: VIC-CCCGAGGGTCCTC-MGB.

[0035] The fluorescent amplification kit of the embodiment of the present invention includes: fluorescent reaction tube, deionized triple distilled water, M...

Embodiment 2

[0036] Example 2, Amplification of oxytocin receptor SNP rs53576

[0037] 1. Extraction of genomic DNA from blood of pregnant women

[0038] The DNA extraction kit used in this example was purchased from Beijing Zhuangmeng International Biogene Technology Co., Ltd. Add 10 μL of proteinase K and 100 μL of the sample to be tested (EDTA anticoagulated whole blood) into a 1.5ml centrifuge tube, mix well, and centrifuge at 2000 rpm For 10 seconds, add 200 μL of buffer B, mix by inverting, place at 56°C for 10 minutes, mix by inverting for 2-3 times during this period, add 200 μL of absolute ethanol, mix by inverting, and add to the adsorption column, centrifuge at 12000rpm for 30 seconds, discard For waste liquid, put the adsorption column back into the collection tube, add 500 μL buffer C to the adsorption column, centrifuge at 12000 rpm for 30 seconds, discard the waste liquid, put the adsorption column back into the collection tube, add 700 μL rinse solution W2 to the adsorption...

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Abstract

The embodiment of the invention discloses a primer group for detecting the SNP genotypes of an oxytocin receptor. The primer group consists of a specific primer pair and probes, wherein the specific primer pair is used for amplifying the SNP rs53576 of the oxytocin receptor; an oxytocin receptor gene is located on 3p25 to 3p26 and comprises four exons and three introns, and the rs53576 is locatedon the third intron; the probes include a first probe with a nucleotide sequence as shown in SEQ ID NO: 3 and a second probe with a nucleotide sequence as shown in SEQ ID NO: 4. According to the embodiment of the invention, the primer group for detecting the SNP genotypes of the oxytocin receptor is provided; the primer pair and the probes are designed for amplifying the SNP rs53576 of the oxytocin receptor; the SNP types of the site SNP rs53576 of the oxytocin receptor of a detected person is judged through a fluorescence signal diagram of an amplification product; and if the SNP type of thesite SNP rs53576 is A / A, the detected person is a normal pregnant woman, or if the SNP type of the site SNP rs53576 is A / G or G / G, the detected person is a pregnant woman related to slow birth process.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of biological detection, in particular to a primer set for detecting oxytocin receptor genotype and its application. Background technique [0002] Since the 1990s, the cesarean section rate in the world has been rising steadily. Among them, "abnormal labor process" is the indication for cesarean section. It is said that most people undergo cesarean section due to stagnant labor. But in the clinical setting, labor may not "stand still" but be slower than expected. If in clinical practice, the characteristics of each patient can be specifically analyzed, unnecessary cesarean section operations can be avoided to a certain extent (second-born scarred uterus, side effects of anesthesia drugs and other cesarean section hazards). A familial association study of dystocia found an association between the oxytocin gene and the oxytocin receptor gene, suggesting that oxytocin plays an import...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 张天舒吕青青张晓静孙丽芳丁楠张洪影徐宇菲郭勇鄂文
Owner 北京德能安康生物科技有限公司
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