LGI 1 gene mutation and application thereof in preparation of temporal lobe epilepsy co-disease depression animal model

An animal model, gene technology, used in applications, genetic engineering, DNA preparation, etc.
CN111004818AActive Publication Date: 2020-04-14NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL +3
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
Publication Date
2020-04-14

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Abstract

The invention relates to the field of gene engineering, and in particular relates to new LGI 1 gene mutation causing temporal lobe epilepsy (TLE) newly found in people and an application. The invention discloses the application of the 152nd gene locus A152G mutation of a first exon of an LGI 1 gene in preparation of a temporal lobe epilepsy co-disease depression animal model, and the application in screening of anti-temporal lobe epilepsy co-disease depression drugs. According to the invention, a homozygous model mouse with the gene mutation shows lethal epilepsy; an electroencephalogram of the heterozygous mouse can record epilepsy waveforms, and behavioristics shows that the mouse has co-disease depression; and the mouse can serve as new animal model of epilepsy co-disease depression andis used for mechanism research of epilepsy attack and epilepsy co-disease depression and research and development of anti-epilepsy combined depression drugs.
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Description

technical field

[0001] The present invention relates to a new LGI1 gene mutation newly discovered in the population that leads to temporal lobe epilepsy (TLE). Homozygous model mice with this gene mutation exhibit lethal epilepsy, and the EEG of heterozygous mice can record epileptic waveforms , Behavioral studies show comorbid depression, which can be used as a new animal model for epilepsy comorbid depression, for research on the mechanism of epilepsy and epilepsy comorbid depression, and for the development of antiepileptic drugs combined with depression. Background technique

[0002] Temporal lobe epilepsy (TLE) is the most common focal epilepsy syndrome, often accompanied by pathological abnormalities such as hippocampal sclerosis, neuron loss, glial cell proliferation, and mossy fiber sprouting Abnormal epileptic discharges originate in the temporal lobe of the brain and are partial A type of epilepsy. Temporal lobe epilepsy is divided into lateral temporal lobe epile...

Claims

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