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A method for extracting flavonoids in wild rice by deep eutectic solvent
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A kind of deep eutectic solvent and medium flavonoid technology, which can be used in anti-toxic agent, drug combination, organic chemistry and other directions, and can solve the problems of flammability, toxic organic solvent and low vapor pressure.
Active Publication Date: 2021-07-23
TOBACCO RES INST CHIN AGRI SCI ACAD
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However, during the extraction process, organic solvents are toxic, have low vapor pressure, are volatile, and are flammable, and are likely to cause pollution to the environment.
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[0041] In the present invention, the preparation method of the deep eutectic solvent preferably includes the following steps: firstly mixing choline chloride and a hydrogen bond donor, and then adding water for second mixing to obtain a deep eutectic solvent. In the present invention, the mode of the first mixing and the second mixing is preferably stirring and mixing; the rotational speed of the stirring and mixing is preferably 200-800 rpm, more preferably 350-550 rpm; the temperature of the first mixing is preferably 45 ~80°C, more preferably 60-80°C, most preferably 80°C; the time of the first mixing is preferably 30-360 min, more preferably 60-240 min; the temperature of the second mixing is preferably 25-35 min. °C, and the time is preferably 10-60 min. In the present invention, the deep eutectic solvent is a stable, uniform and transparent liquid. The deep eutectic solvent provided by the invention is an extractant with strong polarity, which can effectively break the ...
Embodiment 1
[0056] (1) Preparation of deep eutectic solvent
[0057] Choline chloride and 1,4-butanediol were added to a round-bottomed flask in a molar ratio of 1:6, a magnet was added to the round-bottomed flask, and an IKA magnetic stirrer was used for magnetic stirring at 80 °C for 60 min. A choline chloride-1,4-butanediol mixed solution was obtained, and 30 wt % of water was added to the obtained mixed solution to obtain a deep eutectic solvent (abbreviated as DES-1).
[0058] (2) Wild rice pretreatment
[0059] The wild rice was ground with a pulverizer, dried at 40° C. until constant weight, and the obtained wild rice powder was stored at -20° C. in the dark for future use.
[0060] (3) Analysis of wild rice powder
[0061] Using a BSA124S-CW electronic balance, weigh 500 mg of wild rice powder, add 10 mL of deep eutectic solvent and mix thoroughly, and place it in a KQ-500GVDV type double-pin constant temperature numerically controlled ultrasonic generator. Under the condition ...
Embodiment 2～3
[0080] The flavonoids were extracted and detected according to the method of Example 1. The difference from Example 1 was that the deep eutectic solvent was different. The compositions of deep eutectic solvents in Examples 1 to 3 were shown in Table 3. Table 4 shows the extracted amounts of these three flavonoids.
[0081] The composition of deep eutectic solvent in the embodiments 1 to 7 of table 3
[0082]
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Abstract
The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoids in wild rice by using a deep eutectic solvent. The method for extracting flavonoids in wild rice with a deep eutectic solvent provided by the present invention comprises the following steps: pretreating the wild rice to obtain wild rice powder; extracting the wild rice powder with a deep eutectic solvent and solidifying liquid separation to obtain flavonoid extracts; liquid chromatographic separation of the flavonoid extracts to obtain flavonoids; the deep eutectic solvent includes choline chloride, hydrogen bond donors and water; the hydrogen bond donors These include 1,4-butanediol, glycerol or lactic acid. The deep eutectic solvent adopted in the invention is non-toxic, biodegradable, green and environmentally friendly, and can be recycled and reused, thereby greatly reducing the extraction cost. The method provided by the invention has high extraction efficiency and low detection limit for flavonoids in wild rice.
Description
technical field [0001] The invention relates to the technical field of natural substance extraction, in particular to a method for extracting flavonoids in wild rice by using a deep eutectic solvent. Background technique [0002] Wild rice is the caryopsis of Chinese wild rice, a nutrient-rich whole grain that has been eaten in China for at least 3,500 years. It mainly grows in diving swamps, ponds, swamps, paddy fields and shallow lake areas on the edge of lakes; it is widely distributed in Northeast China, North China, Central China, South China, Southwest China, and Central South China. Wild rice contains protein, minerals, vitamins, phenols, saponins, phytosterols and anthocyanins and other phytochemical components. In addition, wild rice has rich biological activities, including antioxidant, anti-inflammatory, anti-obesity and anti-allergic effects, which have attracted extensive attention of domestic and foreign researchers. [0003] At present, flavonoids isolated f...
Claims
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