Molecular marker, primer composition and kit for calculating boettcherisca peregrina larva stage development time, and application

Abstract

The invention discloses a molecular marker, a primer composition and a kit for calculating boettcherisca peregrina larva stage development time, and application, and specifically discloses molecular marker Spprotein singed46.156 target gene. The primer composition comprises singed-F and singed-R. The kit for calculating boettcherisca peregrina larva stage development time comprises a primer composition and a buffer solution. The Spprotein singed46.156 target gene has a potential protein binding function, can be possibly related to synthesis and degradation of a certain protein in the degeneration process of the species, and has remarkable differential expression in different development periods; a molecular genetic marker for larval stage development time calculation is screened out by combining a qRT-PCR technology, and a regression equation of differential expression genes and the development time is constructed to carry out auxiliary judgment of different development stages, the molecular, the primer composition and the kit can be used for calculating the boettcherisca peregrina larva stage development time, and more scientific, objective and fair data reference is provided forPMI inference application in actual cases.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a molecular marker, a primer composition, a kit and an application for estimating the larval development time of the sarcophagus fly. Background technique [0002] Forensic entomology is a branch of forensic science that applies entomological knowledge to solve legal problems. It has unique advantages in inferring the time of death, especially the postmortem interval (PMI) of decayed corpses. Using the morphological changes in the non-adult stages (eggs, larvae, and pupae) of sarcophilus flies combined with environmental temperature and other conditions to determine the developmental stage and calculate the time elapsed to this stage is the main theoretical basis for the existing PMI inference . There are obvious differences in the growth and development rates of necrophagous flies in different environmental temperatures, so the accurate judgment of the developmental stage is t...

AI Technical Summary

Problems solved by technology

In a case report, a decomposing corpse covered with fly maggots was found in a certain water area, and it was later identified that the fly maggots were mainly larvae of B. After investigation, many witnesses confirmed that the time of the incident was about 128 hours, so this species has certain advantages in PMI estimation, but there are still some shortcomings based on the current traditional method (the actual time of the incident is not within the estimated time range, which will cause Investigators delineate suspicious persons committing crimes time deviation)

Images