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Paramyxoviridae expression system

A paramyxovirus and virus technology, applied in the field of vaccines, can solve problems such as loss of inserted genes

Pending Publication Date: 2020-05-01
BOEHRINGER INGELHEIM VETMEDICA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, a disadvantage seen in practice is that this viral strain (possibly mediated by homologous recombination between repetitive noncoding sequences) can lose the inserted gene over time

Method used

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Embodiment Construction

[0026] The present invention solves the problems inherent in the prior art and provides a significant advance over the state of the art.

[0027] The present invention demonstrates the exploration of CDV recombinants (ie, CDV vectors) encoding VP2 proteins of 2b or 2c CPV genotypes generated according to the examples provided herein by in vitro data. The viruses displayed good growth kinetics for production in Vero cells and reached peak titers at 6 days post infection in roller bottles. These recombinants displayed strong expression properties of the transgene (VP2), as determined by immunofluorescence and Western blot, which quantified these recombinants as dual CDV-CPV vaccine candidates. Additionally, the genetic stability of the viruses was tested in Vero cells for 20 cell passages. Both viruses remained completely genetically stable, indicating their ease of bioprocessing for vaccines.

[0028] The present invention further confirms through in vivo results that the CDV...

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Abstract

The present invention relates to the field of (vector) vaccines, and especially to an enhanced arrangement of nucleotide sequences for expressing a Paramyxoviridae virus containing an exogenous gene of interest. The present invention further concerns related expression cassettes and vectors, which are suitable to express genes of interest, especially antigen encoding sequences. The viral vectors of the present invention are useful for producing an immunogenic composition or vaccine.

Description

technical field [0001] The present invention relates to the field of (vector) vaccines, and in particular to the enhanced arrangement of nucleotide sequences for expressing an exogenous gene of interest by a Paramyxoviridae virus containing the exogenous gene. The invention additionally relates to related expression cassettes and vectors suitable for expressing genes of interest, especially antigen coding sequences. The viral vectors of the invention can be used to produce immunogenic compositions or vaccines. Background technique [0002] Paramyxoviruses of the Paramyxoviridae family are negative-sense, single-stranded, RNA viruses responsible for many endemic animal and human diseases. Examples of paramyxoviruses are Newcastle disease virus, which infects poultry and other avian species, or measles virus, which causes disease in humans. The viruses currently comprise 49 species and are divided into 7 genera, of which the Morbillivirus genus has 7 species. [0003] Canin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/155A61K39/175C07K14/115C12N15/86
CPCA61K39/12C12N15/86A61K2039/53A61K2039/552C12N7/00C12N2750/14022C12N2750/14034C12N2760/16022C12N2760/16034C12N2760/18421C12N2760/18434C12N2760/18443C12N2770/20022C12N2770/20034C07K14/115A61K2039/522A61K39/155
Inventor V·尼科林A·本特A·加列
Owner BOEHRINGER INGELHEIM VETMEDICA GMBH
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