Degraded coral reef bioremediation method based on macrocalcified algae
A technology for coral reefs and seaweeds, which is applied in the application field of biotechnology and achieves the effects of a simple transplant method, low transplant cost and simple method.
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[0038] 1. Sample collection and holding
[0039] In order to better reflect the repairing effect of large-scale calcified algae, this experiment selected large-scale calcified seaweeds widely distributed in the coral reef ecosystem of the South China Sea, such as Halimeda, Amphiroa and shell-like coralline algae. According to the shape of the algae, different transplanting and feeding methods were adopted ( figure 1 ). There are two main ways to obtain seedlings, one is through artificial semi-indoor breeding technology; the second is through in-situ sampling in the field to ensure sufficient, stable and healthy sources of algal species. Put the seaweed in 10 250L polyethylene large white buckets in batches and inflate them temporarily. The water temperature is between 27-29°C; the seawater for cultivation is replaced every 2 days. The salinity of the seawater is between 32-34ppt, and the pH range is 8.02 –8.23; the light cycle (Light: Dark) is set to 16L:8D, and the light i...
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