Serological method for identifying sex of gingko seedlings
A technology of serology and ginkgo, applied in the field of biological identification, can solve the problems of high toxicity, unfavorable promotion, complicated operation procedures, etc., and achieves the effect of less operation difficulty and easy mass production.
Active Publication Date: 2020-05-05
NANJING HEJIACHUN BIOTECH CO LTD
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[0004] Aiming at the deficiencies in the prior art, the present invention provides a serological method for discriminating the gender of ginkgo seedlings, which has the advantages of low difficulty in operation, simple and easy operation, high wide applicability, low cost, little pollution and little poisoning, etc., and solves the problem of The existing identification methods generally have complex operation procedures, and the identification of drugs is expensive or toxic, which is not conducive to the promotion of problems
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[0030] For the sake of clarity, many practical details are included in the following narrative. It should be understood, however, that these practical details should not be used to limit the invention. That is, in some embodiments of the invention, these practical details are not necessary.
[0031] In addition, in the present invention, descriptions such as "first", "second" and so on are used for description purposes only, and do not refer to the meaning of order or sequence, nor are they used to limit the present invention, but are only for the purpose of distinguishing the following The components or operations described by the same technical terms are only used, but should not be understood as indicating or implying their relative importance or implying the number of indicated technical features. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the technical solutions of the various embo...
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Abstract
The invention relates to the technical field of biological identification, and particularly discloses a serological method for identifying the sex of gingko seedlings. The serological method comprisesthe following steps: preparing a gingko adult plant tissue extracting solution with known sex; respectively injecting the ginkgo adult plant tissue extracting solution of male and female plants intorabbits for immunization; and extracting rabbit blood to obtain serum, then fully mixing the serum with gingko adult plant tissues of male and female plants with opposite sexes, centrifuging the mixedsolution to obtain specific gingko sex identification antiserum of the male and female plants, and identifying in an agar diffusion agglutination test to obtain the sex of gingko seedlings to be detected. Compared with various existing identification methods, the serological method is low in operation difficulty, simple, convenient and easy to implement, less in use of expensive or highly toxic drugs and easy for mass production.
Description
technical field [0001] The invention relates to the technical field of biological identification, in particular to a serological method for identifying the sex of ginkgo seedlings. Background technique [0002] Ginkgo biloba is a dioecious plant, which is a multi-purpose special economic ecological tree species integrating edible, medicinal, material, health care, ornamental and ecological protection. In intensive orchards, in order to increase the yield of ginkgo fruit, most of them should be female plants, and a small number of male plants are also needed to facilitate pollination and fertilization. In the process of urban greening, because the female Ginkgo biloba seeds will produce a special smell after they mature and fall to rot, only male plants should be planted, and female plants should not be planted. In other words, ginkgoes of different genders have different uses and need to be used properly. Therefore, the early sex identification of ginkgo seedlings has impo...
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IPC IPC(8): G01N33/559
CPCG01N33/559G01N2333/415Y02A50/30
Inventor 汪良驹郝麒汪文韬曲咏梅
Owner NANJING HEJIACHUN BIOTECH CO LTD