Method for realizing rice heterosis fixation

Abstract

The invention discloses a method for realizing rice heterosis fixation. An OsMADS13 promoter and a rice BBM1 gene coding sequence are connected to construct an expression cassette A; a rice zygotic embryo development key gene OsMPK6 double-target CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas9 knockout expression cassette B is constructed; the expression cassette A and theexpression cassette B are integrated to the same expression carrier, the expression carrier is converted into a hybrid rice seed through an agrobacterium-mediated method to obtain a T0-geneation plant; and the T0-geneation plant is subjected to selfing to obtain a selfed seed. According to the method disclosed by the invention, a rice nucellus tissue is used for specifically expressing the promoter, and a rice embryo forming key gene is subjected to specific expression in the hybrid rice nucellus tissue to induce a nucellus tissue somatic cell to form an adventitious embryo; the rice zygoticembryo development key gene is knocked out to damage a sexual reproduction process of the rice; and finally, through hybrid rice selfing, a seed capable of realizing rice heterosis fixation through asomatic cell adventitious embryo way is formed. According to the method, hybrid rice grouping efficiency can be greatly improved, and seed production does not need to be carried out in each year.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for realizing rice heterosis fixation. Background technique [0002] Rice heterosis utilization technology was first successfully applied in my country. After more than 40 years of development, it has made great achievements and provided an important guarantee for my country's food self-sufficiency. At the same time, hybrid rice is also used in Southeast Asia, India, the United States and other countries and regions in the world It has been widely used in production and has been identified by the Food and Agriculture Organization of the United Nations as the preferred technology to solve food shortages in developing countries. Therefore, hybrid rice has and will continue to provide an important guarantee for world food security. The hybrid rice currently used in production is mainly "three-line hybrid rice" based on nuclear-cytoplasmic interaction sterility and "...

AI Technical Summary

Problems solved by technology

[0004] Previous studies have found that adventitious embryonic phenomenon widely exists in rice, which is directly developed through the somatic cells of the nucellus tissue, and the genotype is exactly the same as that of the parent, but because the frequency of occurrence is not high and there is a sexual reproduction mode, it is usually It exists in the form of twin embryos or polyembryonic seedlings, and cannot be used for heterosis fixation in production

In addition, existing studies have also shown that mutations in some genes can lead to abnormal development of rice embryos and the inability to produce seeds.

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