Indirect ELISA detection kit and detection method for detecting chlamydia pneumoniae antibody

Abstract

The invention discloses an indirect ELISA detection kit for detecting a chlamydia pneumoniae antibody and a detection method. The detection kit comprises a chlamydia pneumoniae recombinant antigen ELISA coated ELISA strip, a serum dilution plate, a diluent, a positive control solution, a negative control solution, an enzyme-labeled secondary antibody conjugate, a washing solution, a TMB developingsolution and a stop solution. The stop solution is 2 M concentrated sulfuric acid. The sequence of the CP recombinant antigen is SEQ ID NO. 1. The optimal antigen coating concentration and serum dilution are optimized through a square matrix, the dilution of the enzyme-labeled secondary antibody is determined, and the type and closing time of the confining liquid and the antibody incubation timeare determined. The kit has the advantages of strong specificity, high sensitivity, good stability, high flux and the like when being used for detecting the CP antibody, can well detect the CP antibody in human serum, and has certain guiding significance for clinical diagnosis.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an indirect ELISA detection kit for detecting chlamydia pneumoniae antibody and a detection method thereof. Background technique [0002] Chlamydia pneumonia (CP) is one of the main pathogens of community-acquired pneumonia, which is relatively common in the world. Young children and the elderly are susceptible groups. The disease can occur in all seasons, and it is mainly transmitted through droplets. Clinically, the main symptoms are respiratory symptoms, sore throat, hoarseness, runny nose, etc., and fever and cough may also be manifested. [0003] CP infection is usually a mild, self-limiting disease that is often overlooked or underdiagnosed because of its atypical symptoms. Studies have shown that CP infection is related to some chronic diseases, such as asthma, otitis media, erythema nodosum, atherosclerosis and endocarditis, coronary heart disease, etc. CP infection c...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide an indirect ELISA detection kit for detecting Chlamydia pneumoniae antibody and its detection method, which can solve the problems of high detection cost, limited sensitivity, poor specificity, high requirements for detection environment, long incubation time, and false positive rate of PCR at present. Difficult to control and other technical issues

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