Plasma miRNA combination for predicting ionizing radiation damage degree

A radiation damage, plasma technology, used in DNA/RNA fragments, recombinant DNA technology, determination/inspection of microorganisms, etc.

Active Publication Date: 2020-05-26
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if it is not possible to determine whether the expression of these miRNAs has an expression-damage effect relationship with the degree of radiation damage, and an expression time-damage effect relationship in the early stage of irradiation, then it cannot be taken for granted that they can be used as early radiation damage markers

Method used

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  • Plasma miRNA combination for predicting ionizing radiation damage degree
  • Plasma miRNA combination for predicting ionizing radiation damage degree
  • Plasma miRNA combination for predicting ionizing radiation damage degree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1. With the increase of irradiation dose, the body weight of mice gradually decreased

[0058] 6-8 weeks C57BL / 6 mice, 20, were randomly divided into control group (NC), different irradiation dose groups (1, 4, 7Gy) ( 60 Coγ-rays), 5 rats in each group. The body weight of the mice was weighed on the seventh day after irradiation.

[0059] The result is as figure 1 shown. It shows that with the increase of the irradiation dose, the body weight of the mice gradually decreases, indicating that the damage is gradually aggravated. Among them, the 7Gy irradiation group was significantly different from the control group (**P<0.01).

Embodiment 2

[0060] Example 2. With the increase of irradiation dose, WBC and PLT in the peripheral blood of mice gradually decreased

[0061] 20 C57BL / 6 mice at 6-8 weeks were randomly divided into control group (NC), different irradiation dose groups (1, 4, 7Gy) ( 60 Coγ-rays), 5 rats in each group. On the seventh day after irradiation, blood was collected through the tail vein to detect the number of white blood cells (WBC) and platelets (PLT) in the peripheral blood of the mice.

[0062] The result is as figure 2 As shown, it is shown that with the increase of the irradiation dose, the number of white blood cells (WBC) and platelets (PLT) in the peripheral blood of mice gradually decreased, indicating that the damage gradually increased. There were significant differences between each radiation dose and the control (*P<0.05; **P<0.01; ***P<0.001).

Embodiment 3

[0063] Example 3: Anti-radioactive drugs WR-2721 and 523 can significantly reduce radiation damage under the same irradiation dose

[0064] 20 C57BL / 6 mice at 6-8 weeks were randomly divided into control group (NC), irradiation group (8Gy, 60 Coγ-rays), the amifostine (WR2721) group was irradiated (150mg / kg WR-2721 was injected intraperitoneally 30min before 8Gy irradiation), and the nilestriol (523) group was given nylestriol (523) before irradiation (10mg / kg intragastric administration 48h before irradiation) kg 523), 5 in each group. On the seventh day after irradiation, blood was collected through the tail vein to detect the number of white blood cells (WBC) and platelets (PLT) in the peripheral blood of the mice.

[0065] The anti-radiation drug Nylestriol (523) was prepared by the Institute of Radiation Medicine, Academy of Military Medicine, Academy of Military Sciences, and Amifostine (WR-2721) was obtained from Dalian Merro Pharmaceutical Factory.

[0066] The resul...

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Abstract

The invention discloses a plasma miRNA combination for predicting the ionizing radiation damage degree. The invention provides an application of all or part of four miRNAs as biomarkers in (A1) or (A2) as follows: (A1) preparation of a product for evaluating radiation damage conditions, and (A2) evaluation of the radiation damage condition; and the four miRNAs are miR-22-5p, miR-30a-3p, miR-375-3pand miR-379-5p. The combined expression of the four miRNAs is mainly related to the damage degree of the radiated tissue and is irrelevant to the radiation irradiation dose, so that the miRNA combination is a plasma molecular marker combination related to ionizing radiation damage. The plasma miRNA combination is of great significance in quickly evaluating the radiation damage degree of large-scale irradiated people in the early stage of irradiation after nuclear explosion or nuclear accidents so as to perform classified rescue and treatment and determine a treatment scheme.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plasma miRNA combination for predicting the degree of ionizing radiation damage. Background technique [0002] Nuclear explosions or nuclear accidents may cause a large number of people to be exposed to radiation. In the initial stage of exposure (within 12 hours), quickly and accurately estimate the dose and radiation damage suffered by the exposed people, and provide a basis for the rapid classification of injuries and the selection of treatment measures for the wounded. , is the key to nuclear accident emergency rescue. In recent years, the ionizing radiation biological dose estimation methods that have been applied at home and abroad mainly focus on cytogenetic methods such as chromosome aberration analysis and micronucleus analysis. It is difficult to meet the needs of rapid and high-throughput dose estimation. [0003] In addition, due to factors such as the complexity of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 葛常辉付汉江郑晓飞朱捷于海月
Owner ACADEMY OF MILITARY MEDICAL SCI
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