Application of ginseng PgWRKY4X transcription factor in regulating and controlling content of ginsenoside compounds in ginseng

A technology of ginsenosides and transcription factors, applied in the field of biochemistry

Active Publication Date: 2020-06-02
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The synthesis of ginsenosides in ginseng is regulated by transcription factors, and there are still some problems in the analysis of its regulatory mechanism. Therefore, it is of great significance to study the regulat

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  • Application of ginseng PgWRKY4X transcription factor in regulating and controlling content of ginsenoside compounds in ginseng
  • Application of ginseng PgWRKY4X transcription factor in regulating and controlling content of ginsenoside compounds in ginseng
  • Application of ginseng PgWRKY4X transcription factor in regulating and controlling content of ginsenoside compounds in ginseng

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention is not limited thereto.

[0024] 1. Experimental materials and instruments

[0025] Plant total RNA extraction kit (B511311, Shanghai Sangon Bioengineering Co., Ltd., China), first-strand cDNA synthesis kit (B639252, Shanghai Sangon Bioengineering Co., Ltd., China), ginsenoside Rb 1 , Rg 1 , Re standard (Dalian Meilun Biotechnology Co., Ltd.). Fluorescence microscope (LEICADM5000B, Buffalo Grove, IL, France).

[0026] 2. Experimental method

[0027] 1. Construction of a recombinant vector containing the gene encoding the ginseng PgWRKY4X transcription factor

[0028] Construction of overexpression recombinant vector containing ginseng PgWRKY4X transcription factor coding gene:

[0029] By PCR technology, using specific primers PgWRKY4X-F-R (SEQ ID No.4 and SEQ ID No.5 in Table 1), using the cDNA obtai...

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Abstract

The invention discloses an application of a ginseng PgWRKY4X transcription factor in regulating and controlling the content of ginsenoside compounds in ginseng. The amino acid sequence of the ginsengPgWRKY4X transcription factor is as shown in SEQ ID No. 1. Experiments prove that the ginseng PgWRKY4X transcription factor can be combined with a promoter of a ginseng PgSE gene, so the expression ofa key enzyme PgSE on a ginsenoside synthesis pathway is improved, and the content of ginsenoside compounds in ginseng is further improved. The ginseng PgWRKY4X transcription factor, an encoding geneof the ginseng PgWRKY4X transcription factor and an overexpression recombinant vector containing the encoding gene can be used for regulating and increasing the content of ginsenoside compounds in ginseng.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to plant gene regulation secondary metabolites, in particular to the application of ginseng PgWRKY4X transcription factor and coding gene in regulating the content of ginsenoside compounds in ginseng. Background technique [0002] Ginseng (PanaxginsengC.A.Mey) belongs to the perennial herb of Araliaceae. In China, ginseng has always been regarded as the king of all herbs. As a traditional and precious Chinese herbal medicine, ginseng has rich pharmacological effects, including antidiabetic activity, antistress activity, antioxidant capacity, immune system regulation and anticancer activity, etc. Among them, what plays an important role is the triterpenoid compound ginsenoside. Ginsenosides in ginseng are divided into three categories according to structural differences: one is protopanaxadiol-type saponins, including ginsenoside Rb 1 , Rb 2 , Rc, Rd, Rg 3 , Rh 2 ; A class of protopanax...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/00
CPCC07K14/415C12N15/8243
Inventor 高文远王娟姚陆贺军平刘静静许馨丹
Owner TIANJIN UNIV
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