Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Target gene capable of improving regeneration capacity of plants, regulation molecules and application of target gene

A regenerative capacity, plant technology, applied in the field of botany, can solve the problem of not meeting the needs of explaining the mechanism of plant regeneration

Active Publication Date: 2020-06-05
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these findings are not enough and cannot meet the needs of explaining the mechanism of plant regeneration, and further exploration is still needed in this field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Target gene capable of improving regeneration capacity of plants, regulation molecules and application of target gene
  • Target gene capable of improving regeneration capacity of plants, regulation molecules and application of target gene
  • Target gene capable of improving regeneration capacity of plants, regulation molecules and application of target gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0072] As an embodiment of the present invention, the expression of the HAM gene in plants can be down-regulated by knocking out the HAM gene.

[0073] As an embodiment of the present invention, the CRISPR / Cas9 system can be used for gene editing, thereby knocking out the HAM gene and improving plant regeneration ability. Appropriate sgRNA target sites will lead to higher gene editing efficiency, so it is more important to design and find appropriate target sites before proceeding with gene editing. After designing specific target sites, in vitro cell activity screening is required to obtain effective target sites for subsequent experiments.

[0074] As an embodiment of the present invention, virus-induced gene silencing (VIGS) can be used to inhibit HAM, thereby improving plant regeneration ability.

[0075] It should be understood that after knowing the correlation between HAM and plant traits, those skilled in the art can prepare molecules that down-regulate HAM in various...

Embodiment 1

[0143] Example 1, miR171 and its target gene HAM are conserved in all plants

[0144] The target genes of miR171 are GRAS transcription factors, including three genes in the Arabidopsis genome, namely HAM1, HAM2 and HAM3.

[0145] Evolutionary analysis showed that miR171 is conserved in all terrestrial plants and widely exists in bryophytes, ferns, gymnosperms and angiosperms, such as figure 1 .

[0146] At the same time, the present inventors used the protein sequence "blast" function of the NCBI website (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome) to search for the complete sequence of HAM3 in Arabidopsis Long protein sequence, obtained protein sequences highly homologous to the Arabidopsis HAM3 protein sequence in several different species, and obtained the sequence information through MAFFT software (https: / / mafft.cbrc.jp / alignment / software / ) analysis After obtaining the homology information, some homology information...

Embodiment 2

[0147] Example 2, miR171 regulates Arabidopsis shoot regeneration ability

[0148] There are three coding genes for miR171 in the Arabidopsis genome, MIR171A, MIR171B, and MIR171C; their coding products, namely mature sequences, are miR171a, miR171b, and miR171c, respectively, and the mature sequences of miR171b and miR171c are the same. The present inventors prepared mir171a, mir171b and mir171ab mutants respectively, and investigated the shoot regeneration ability of the mutants.

[0149] The result is as image 3 As shown, the shoot regeneration ability of mir171b and mir171a b was significantly reduced, suggesting that miR171 is mainly involved in the regulation of shoot regeneration ability.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a target gene capable of improving regeneration capacity of plants, regulation molecules and application of the target gene and discloses a new target HAM (hairy meristem). MiRNA targeted to HAM plays a role of promoting plant regeneration by inhibiting HAM. The new technology which has universality and is capable of increasing regeneration rate of the plants effectively and conveniently, a new way is provided for improved breeding of the plants, and good application prospects are achieved.

Description

technical field [0001] The invention belongs to the field of botany, and more specifically, the invention relates to a target gene for improving plant regeneration ability, a regulatory molecule and application thereof. Background technique [0002] Regeneration refers to the process in which the whole body or organ of an organism is partially lost due to trauma, and on the basis of the remaining part, it grows the same structure as the lost part in terms of shape and function. Among animals, hydra, planarians, echinoderms (starfish, crinoids) and other species can regenerate a complete form at the wound after cutting their body parts. Plant cells are also totipotent, and isolated plant organs (such as roots, hypocotyls, and leaves) can be regenerated by tissue culture. For example, take a branch from a willow tree, cut it up and down, and insert the lower end into moist sand or water. After a period of time, buds and adventitious roots will grow from the upper and lower cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/82C12N5/10A01H5/00A01H6/46
CPCC12N15/8259C12N15/8216C12N5/04A01H5/00A01H6/46C12N15/82C12N5/10Y02A40/146
Inventor 王佳伟吴连宇王龙
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com