Application of pyrazine quinazoline diketone alkaloid compound in preparation of marine fouling organism control agent
A technology of pyrazinquinazoline dione and biological control agent, which is applied in the field of natural products and achieves the effects of good application prospect, great promotion and application potential, and environmental friendliness.
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Embodiment 1
[0020] Experimental group: pyrazine quinazoline dione alkaloid compound ((1S,4S)-4-benzyl-1-hydroxyl-1,2-dihydro-6H-pyrazine[2,1-b]-quinone Azoline-3,6(4H)-dione) was dissolved in methanol to a solution concentration of 282.6 μg / mL, and 1 mL of the solution was added to a petri dish with a diameter of 6 cm to evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the compound coated on the bottom of the petri dish is 10 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0021] Control group: Add 1mL solvent methanol to make it evenly distributed on the bottom of the culture dish, and then add 13mL filtered and sterilized seawater after the volatilization is complete.
[0022] Blank group: add 13mL filtered and sterilized seawater.
[0023] Four parallel samples were set up in each of the experimental group, the blank group and the control group, and about 30 netted barnacle Venus larvae were added respectively. Place them in...
Embodiment 2
[0028] Experimental group: pyrazine quinazoline dione alkaloid compound ((1S,4S)-4-benzyl-1-hydroxyl-1,2-dihydro-6H-pyrazine[2,1-b]-quinone Azoline-3,6(4H)-dione) was dissolved in methanol to prepare a solution with a concentration of 28.26 μg / mL. Add 1 mL of the solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the compound coated on the bottom of the culture dish is 1.0 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0029] Control group: Add 1mL solvent methanol to distribute the solution evenly on the bottom of the petri dish. After the solvent evaporates completely, add 13mL filtered and sterilized seawater.
[0030] Blank group: add 13mL filtered and sterilized seawater.
[0031] Four parallel samples were set up in the experimental group, the blank group and the control group, and about 30 larvae were added to each. Cultivate in a dark environment i...
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