Single-objective lens light-sheet fluorescence microscopy imaging device and method based on micromirror array

Abstract

The invention discloses a single objective lens light sheet fluorescence microscopic imaging device and method based on a micro-mirror array. According to the invention, the micromirror array is utilized, a same objective lens is adopted to combine light sheet excitation and fluorescence collection into one, sample freedom is realized at a sample, complex treatment on the sample is not needed, theinvention can be realized by utilizing a conventional sample glass slide and a cover glass, and light path adjustment is avoided. Micromirror arrays are used so that light sheet excitation fluorescence information of a sample at different depths is reflected by different positions of the micro-mirror array system; imaging is carried out at different positions of a signal collection system, single-objective-lens light sheet fluorescence microscopic imaging is realized, and a large-numerical-aperture objective lens can be used, so that the limitation that the resolution cannot be further improved in traditional light sheet fluorescence microscopic imaging is improved; in addition, the optical path design is simple and easy to implement, and subsequent industrial development is convenient to achieve.

Description

technical field [0001] The invention relates to light-sheet fluorescence microscopic imaging technology, in particular to a micromirror array-based single-objective light-sheet fluorescent microscopic imaging device and an imaging method thereof. Background technique [0002] With the continuous development of fluorescent protein and staining technology, fluorescence microscopy technology can realize the specific labeling of various molecules and organelles in cells, so as to restore the fine structure in cells, and track and detect individual molecules or organelles. Then realize the exploration and regulation of the essence of life. In the fluorescence microscope, the excitation light is used to illuminate the area to be observed of the entire sample. While obtaining the three-dimensional distribution of the target, it is necessary to expose the fluorescent group multiple times. Therefore, since the development of fluorescence technology, there have been two problems, name...

AI Technical Summary

Problems solved by technology

The optical path design where the excitation objective lens and the collection objective lens are placed vertically, due to consideration of the mechanical structure and working distance of the objective lens, will limit the use of large numerical aperture objective lenses in the imaging system, and fundamentally limit the improvement of system resolution.

In order to solve this problem, some single-objective light-sheet fluorescence microscopy imaging methods have been proposed, but these methods require special treatment of the sample cavity, or a complex and precise adjustment of the optical path to achieve

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