Application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease
A technology for ischemic heart disease and reperfusion injury, which is applied in the field of biomedicine and can solve the problems that have not yet been reported on the application of benzamine in ischemic heart disease.
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[0024]The following examples are provided to further illustrate but not limit the present invention.
[0025] 1. Materials and methods:
[0026] 1.1 Rat cardiomyocytes
[0027] Clean-grade newborn male wistar rat cardiomyocytes within 3 days can be obtained after primary culture.
[0028] 1.2 Reagents
[0029] DMEM-H medium (Gibco, U.S.), DMEM-L medium (Gibco, U.S.), fetal bovine serum (Gibco, U.S.), trypsin (Gibco, China), penicillin-streptomycin solution 100X (Gibco, U.S.) , China), PBS (Beijing Zhongshan), Brdu (Gibco, USA), CCK-8 kit (Beyond, China), benzamine (MedChemExpress, China) and metoprolol (Selleck, China).
[0030] 1.3 Instruments
[0031] Biological safety cabinet (BSC-1300ⅡA2, Shanghai Boxun Industrial Co., Ltd.), ultra-clean bench (SW-CJ-2F, Shanghai Boxun Industrial Co., Ltd.), inverted microscope (ECLIPSE Ti-s Nikon, Japan), upright microscope (CX22OLYMPUS , Japan), 0.22um mixed cellulose filter membrane (MILLIPORE, USA), carbon dioxide incubator (Sanyo...
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