Application of tanespimycin to preparation of antibacterial and anti-inflammatory medicament

A temsiromycin, antibacterial and anti-inflammatory technology, applied in the field of biomedicine, can solve the problems that there are no reports on the application of temsiromycin antibacterial and anti-inflammatory drugs

Inactive Publication Date: 2019-04-05
GENERAL HOSPITAL OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, according to the prior art, temsiromycin is mainly used in the preparation of antitumor drugs, and there is no report on the application of temsiromycin in the preparation of antibacterial and anti-inflammatory drugs

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  • Application of tanespimycin to preparation of antibacterial and anti-inflammatory medicament
  • Application of tanespimycin to preparation of antibacterial and anti-inflammatory medicament
  • Application of tanespimycin to preparation of antibacterial and anti-inflammatory medicament

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Embodiment Construction

[0023] The following examples are provided to further illustrate but not limit the present invention.

[0024] 1. Materials and methods:

[0025] 1.1 Bacteria and cells

[0026] Fusobacterium nucleatum (F. nucleatum, Fn, ATCC 25586) was obtained from the frozen bacterial strain bank of the Key Laboratory of Oral Tissue Regeneration in Shandong Province.

[0027] Gingival fibroblasts (HGF): isolated from 6 volunteer patients aged 18-30 who underwent extraction of impacted teeth.

[0028] 1.2 Reagents

[0029] Defibrinated sheep blood (Haibo Bio Company, Qingdao, Shandong, China), PBS (Soleibao, Beijing, China), hemin-vitamin K1 (Haibo Bio Company, Qingdao, Shandong, China), brain Heart infusion blood culture medium (Suolaibao, Beijing, China), BHI liquid medium (Suolaibao, Beijing, China), type I collagenase (Suolaibao, Beijing, China), DispaseII dispase (Invitrogen, Carlsbad, CA, USA), intracellular reactive oxygen species detection kit (Bebo, Shanghai), temspirulina (Sell...

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Abstract

The invention discloses application of tanespimycin to preparation of an antibacterial and anti-inflammatory medicament. RNA-seq transcriptome analysis is performed on cells of a person suffering frombacterial infection to obtain a list of candidate medicaments with antibacterial and anti-inflammatory effects based on a co-expression path and a medicament gene enrichment analysis method. The listof candidate medicaments is found to include a plurality of commercially-available medicaments with the antibacterial and anti-inflammatory effects, the tanespimycin of which the antibacterial and anti-inflammatory effects is not found before is in the list of candidate medicaments, and the effect of the tanespimycin is verified to be equivalent to the effects of common antibacterial and anti-inflammatory medicaments metronidazole and gentamicin on the cell level, so that the tanespimycin can be applied to the preparation of the antibacterial and anti-inflammatory medicaments.

Description

technical field [0001] The invention relates to new uses of medicines, in particular to the application of tamsiromycin in the preparation of antibacterial and anti-inflammatory drugs, and belongs to the field of biomedicine. Background technique [0002] Tamspirulina (see Tanespimycin, 17-N-allylamino-17-demethoxygeldanamycin, 17-AAG) is a derivative of the antibiotic geldanamycin that is being investigated for the treatment of cancer, particularly in people with certain types of leukemia or In young patients with solid tumors, especially renal tumors. [0003] Tanespimycin exerts a tumor-suppressing effect by inhibiting Hsp90 (see Dimopoulos M, Mitsiades CS, Anderson KC, Richardson PG: Tanespimycin as Antitumor Therapy. Cl Lymph Myelom Leuk 2011, 11:17-22.). Tamsiromycin is an effective Hsp90 inhibitor with an IC50 of 5nM, and its binding affinity to HSP90 in tumor cells is 100 times higher than that in normal cells. It belongs to the class of antitumor antibiotics. Tam...

Claims

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Application Information

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IPC IPC(8): A61K31/395A61P31/04A61P29/00
CPCA61K31/395A61P29/00A61P31/04
Inventor 何昆仑贾志龙冯强康文燕王卫东石金龙赵晓静贾倩刘春蕾
Owner GENERAL HOSPITAL OF PLA
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