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A pertussis filamentous hemagglutinin fusion protein and its application

A fusion protein, pertussis technology, applied in the direction of immunoglobulin, antibacterial immunoglobulin, hybrid peptide, etc., can solve problems such as child death

Active Publication Date: 2022-05-03
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite the availability of a preventive vaccine against pertussis and high vaccination coverage, pertussis remains one of the leading causes of death in children

Method used

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  • A pertussis filamentous hemagglutinin fusion protein and its application
  • A pertussis filamentous hemagglutinin fusion protein and its application
  • A pertussis filamentous hemagglutinin fusion protein and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0069] Embodiment 1 prevents the preparation of animal pertussis vaccine

[0070] The present invention relates to the gene of 3 proteins, respectively the nucleotide sequence is His-StxB-Fha of SEQ ID No.1 1877-2250 Gene (coding amino acid sequence is the protein of SEQ ID No.2

[0071] His-StxB-Fha 1877-2250 ), the nucleotide sequence is the StxB-Fha of the 109-1509th position of SEQ ID No.1 1877-2250 Gene (the coding amino acid sequence is the protein StxB-Fha of the 37th-503rd position of SEQ ID No.2 1877-2250 ) and His-Fha as a control 1877-2250 Gene (coding protein His-Fha 1877-2250 ).

[0072] SEQ ID No.1 consists of 1536 nucleotides, the 1-108th is the sequence on pET28a(+), the 109-378th is the StxB gene, the 379-390th is the connecting peptide gene, and the 391-1509th Fha 1877-2250 Gene, 1510-1536 is the sequence on pET28a(+). a) The gene name is His-StxB-Fha 1877-2250 Gene, the gene name of b) is StxB-Fha 1877-2250 Gene.

[0073] Among them, SEQ ID No.2 c...

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Abstract

The present invention relates to a pertussis filamentous hemagglutinin fusion protein and its application. The fusion protein is composed of pertussis FHA amino acid residue 1877-2250 fragment and StxB through peptide chain connection, and the fusion protein is used to construct a subunit vaccine, and The expression was successfully induced in Escherichia coli, and the immunogenicity of the fusion protein was identified by Western-blot and ELISA; after immunization of mice, it was found that a high level of antibody titer could be produced, which provided a new idea for the research of pertussis vaccine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a pertussis filamentous hemagglutinin fusion protein and its application. Background technique [0002] Pertussis, an acute respiratory infectious disease caused by Bordetella pertussis, is one of the relatively new human infectious diseases. The disease, which first appeared in France in 1414, is one of the world's worst preventable diseases with vaccines. Pertussis is clinically characterized by paroxysmal or explosive multiple rapid coughs, accompanied by a characteristic "chicken crowing"-like inspiratory roar, and the most common complication is secondary pneumonia. The disease is more common in children and infants, especially those younger than 6 months. Despite the availability of a preventive vaccine against pertussis and high vaccination rates, pertussis remains one of the leading causes of death in children. [0003] Natural filamentous hemagglutinin (filamentous haemag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70C12N1/21C07K16/12A61K39/10A61P31/04A61P11/00
CPCC07K14/235C12N15/70C07K16/1225A61K39/099A61P31/04A61P11/00C07K2319/21
Inventor 王恒樑朱力潘超刘兆禄吴军孙鹏王斌曾明冯尔玲
Owner ACADEMY OF MILITARY MEDICAL SCI