Production process of bovine collagen peptides

A production process and original protein technology, which is applied in the production process of cowhide collagen peptides, can solve the problems of irreversible loss of products, unpleasant sensory perception, deepening of product color, etc., achieve high biological antioxidant activity, avoid loss of protein peptides, The effect of reducing the quality difference

Active Publication Date: 2020-06-26
湖北瑞邦生物科技有限公司
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AI Technical Summary

Benefits of technology

This patented method allows cows' skin or blood plasma (BP) samples to be treated beforehand without compromising their qualification. It does this by breaking down large amounts of these components with different techniques like sonolysis, heat treatment, acid/base treatments, etc., which helps them break up connections within each component material better than traditional methods such as boiling water extraction. By doing this, they become clearer images on electron microscopy, allowing researchers to study how tissue changes during disease processes occur over longer periods of time compared to older methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the stability and functionality of collagopepeptides during storage at high temperatures without losing their effectiveness when removed with activated charcoal powders that may leave behind unwanted odors.

Method used

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  • Production process of bovine collagen peptides
  • Production process of bovine collagen peptides
  • Production process of bovine collagen peptides

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A kind of production technology of cowhide collagen protein peptide, comprises the following steps:

[0040] S1 Cleaning: Wash 100 parts of cowhide with water, immerse the cleaned cowhide in 1400 parts of water, add NaOH to adjust the pH value to 13, add 1.35 parts of H 2 o 2 , soak for 78 hours;

[0041] S2 Denaturation: Wash the clean cowhide obtained in step S1 until the pH is neutral, then cut and grind it to make a cowhide slurry, add water 5 times the mass fraction of the cowhide slurry to the cowhide slurry, and set the heat preservation temperature during denaturation to 72°C, the holding time is set to 5.5h, and the denatured cowhide protein slurry is obtained;

[0042] S3 Enzymolysis: Add 3.75 parts of alkaline protease to the denatured cowhide protein slurry obtained in step S2 denaturation, keep the temperature at 57°C, keep the pH at 8.2, and react for 5.5 hours. The power is 700kW, and finally the enzymolysis solution is obtained;

[0043] S4 centrifug...

Embodiment 2

[0048] A kind of production technology of cowhide collagen protein peptide, comprises the following steps:

[0049] S1 Cleaning: Wash 100 parts of cowhide with water, immerse the cleaned cowhide in 1000 parts of water, add NaOH and NaOH 2 CO 3 Adjust the pH value to 10, add 2 parts of stable chlorine dioxide, and soak for 50 hours;

[0050] S2 Denaturation: Wash the clean cowhide obtained in step S1 until the pH is neutral, then cut and grind it to make a cowhide slurry, add water 4 times the mass fraction of the cowhide slurry to the cowhide slurry, and set the heat preservation temperature during denaturation to 60-80°C, the holding time is set to 6h, and the denatured cowhide protein slurry is obtained;

[0051] S3 Enzymolysis: Add 2 parts of alkaline protease to the denatured bovine skin protein slurry obtained in step S2 denaturation, keep the temperature at 65°C, keep the pH at 7.5, and react for 6 hours. The power is 300kW, and finally the enzymatic hydrolysis soluti...

Embodiment 3

[0057] A kind of production technology of cowhide collagen protein peptide, comprises the following steps:

[0058] S1 Cleaning: Wash 100 parts of cowhide with water, immerse the cleaned cowhide in 2000 parts of water, add NaOH and CaO to adjust the pH value to 14, add 0.5 part of sodium hypochlorite, and soak for 200 hours;

[0059] S2 Denaturation: Wash the clean cowhide obtained in step S1 until the pH is neutral, then cut and grind it to make a cowhide slurry, add 6 times the mass fraction of the cowhide slurry to the cowhide slurry, and set the heat preservation temperature during denaturation to 80°C, the holding time is set to 4h, and the denatured bovine hide protein slurry is obtained;

[0060] S3 Enzymolysis: Add 5 parts of alkaline protease to the denatured cowhide protein slurry obtained in step S2 denaturation, keep the temperature at 50°C, keep the pH at 9.5, and react for 5 hours. Use ultrasonic-assisted enzymolysis during the enzymolysis process. The power is ...

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Abstract

The invention belongs to the technical field of enzymolysis of bovine collagen peptides, and particularly relates to a production process of bovine collagen peptides. The production process comprisesthe following steps of: S1, cleaning; S2, denaturation; S3, enzymolysis; S4, centrifugation; S5, enzyme deactivation; S6, impurity removal; and S7, finished product obtaining. The production process has the following advantages and effects: the application adopts the combined action of an ultrasonic process, a denaturation process and an enzymolysis process, and can be conveniently adapted to cowleather in various production places; the collagen peptide product produced by the process has good solubility and dispersibility, good sensory quality and high yield, and a large amount of active polypeptides in the product enable the product to have strong antioxidant capacity; and the method provided by the invention is relatively mild in reaction conditions and low in production energy consumption, and the process conditions and the process effect are far superior to those of the prior art scheme.

Description

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Claims

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Application Information

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Owner 湖北瑞邦生物科技有限公司
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