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Application of NRF2 protein in preparation of drug for regulating biorhythm

A biorhythm, 1. NRF2 technology, applied in the field of regulating biorhythm, can solve the problems of slow response and easy interference of biorhythm regulation technology, and achieve the effect of rapid regulation without interference

Pending Publication Date: 2020-07-03
63919 TROOPS PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The purpose of the present invention is to provide the application of NRF2 protein in the preparation of medicines for regulating biological rhythm in view of the defects of slow response and easy interference of biological rhythm regulation technology in the above-mentioned prior art

Method used

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  • Application of NRF2 protein in preparation of drug for regulating biorhythm
  • Application of NRF2 protein in preparation of drug for regulating biorhythm
  • Application of NRF2 protein in preparation of drug for regulating biorhythm

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The purpose of the study on the regulation of NRF2 protein on biological rhythms is to study NRF2 by reducing the synthesis of NRF2 protein, promoting the decomposition of NRF2 and increasing the synthesis of NRF2 by taking the mouse embryonic fibroblast cell line NIH / 3T3 cells as the research object. The expression and rhythmic changes of biological rhythm proteins CLOCK and BMAL1 were studied to study the influence of NRF2 protein on biological rhythm.

[0050] The process and results are shown below.

[0051] 1. Reducing the synthesis of NRF2 can regulate the content of rhythmic proteins CLOCK and BMAL1:

[0052] 1. NRF2 siRNA screening that can reduce NRF2 synthesis:

[0053] a. Experimental method:

[0054] RNA interference technology is a common technique to reduce protein synthesis. Through cell transfection technology, the RNA interference agent siRNA is completely paired with the messenger RNA corresponding to the target protein to inhibit the transcription ...

Embodiment 2

[0169] The regulation and mechanism of bortezomib BTZ on circadian rhythm, the purpose of which is to use the mouse embryonic fibroblast cell line NIH / 3T3 cells as the research object, use bortezomib BTZ to activate NRF2, and use all-trans retinoic acid ATRA to inhibit NRF2 To study the effect of bortezomib on the content and rhythm of biological rhythm proteins CLOCK and BMAL1; to study the molecular mechanism of bortezomib to regulate biological rhythm by detecting the combination of NRF2 protein with Clock and Bmal1 genes.

[0170] The process and results are shown below.

[0171] 1. BTZ can regulate the content of rhythmic proteins CLOCK and BMAL1:

[0172] 1. Effects of different concentrations of BTZ and ATRA on NRF2 activity:

[0173] a.Experimental method

[0174] After the NRF2 protein is activated (not necessarily increased in content), it enters the nucleus, combines with downstream genes, promotes the expression of these genes and protein synthesis, thereby incre...

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Abstract

The invention discloses an application of NRF2 protein in preparation of a drug for regulating biorhythm. An NRF2 agonist is bortezomib. The application has the beneficial effects that rapid adjustment and no-interference adjustment of the biorhythm can be realized; specifically, the application is implemented in that 1) the NRF2 protein is directly activated, so that the NRF2 rapidly enters a cell nucleus to play a role, so that a slow-speed adjustment path by increasing the content of the NRF2 protein in a DNA-mRNA-protein process is avoided; and 2) the content of the biorhythm protein is directly increased by promoting the combination of the NRF2 protein and a biorhythm related gene, so that the biorhythm is rapidly adjusted, a slow-speed adjustment path by increasing the content of thebiorhythm content indirectly through a biorhythm feedback protein is avoided, and the interference of the biorhythm feedback protein on the biorhythm protein content is also avoided.

Description

technical field [0001] The invention relates to the technical field of regulating biological rhythm, in particular to the application of NRF2 protein in the preparation of medicines for regulating biological rhythm. Background technique [0002] Biorhythm is a cycle of life activity phenomena in a certain chronological order and shown at various levels in the body, such as molecules, cells, organs, systems, and behaviors. Circadian rhythms regulate a wide variety of physiological functions, such as body temperature, cardiovascular activity, respiration, immune response, hormone secretion, behavior, and cognitive functions. [0003] The core mechanism of circadian rhythm is the molecular oscillation formed by the transcription and post-transcriptional regulation of circadian rhythm genes. CLOCK, BMAL1 protein is the molecular mark and core of biological rhythm. After the rhythm gene is activated, the corresponding protein is generated through transcription and translation. ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K38/05A61P43/00A61P9/00A61P11/00A61P37/02A61P5/00A61P25/28
CPCA61K38/16A61K38/05A61P43/00A61P9/00A61P11/00A61P37/02A61P5/00A61P25/28A61K2300/00
Inventor 陈海龙曲丽娜李莹辉吕柯费俊龙冀国华元艳宏卢亮梁峰吉李铠
Owner 63919 TROOPS PLA
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