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A method for extracting tryptophan from fermentation broth

A fermentation broth, tryptophan technology, applied in the direction of organic chemistry, can solve the problems of increasing the burden of subsequent processes, increasing heat energy consumption, prolonging treatment time, etc., to reduce water resources and heat energy consumption, reduce sewage discharge, and reduce treatment. amount of effect

Active Publication Date: 2022-04-15
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the content of tryptophan in the fermentation broth, a large amount of water needs to be added to dissolve the crystals, which will increase the water consumption, increase the volume of materials processed in the subsequent process, increase the burden of the subsequent process, and prolong the processing time. Increase heat energy consumption, increase production cost

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  • A method for extracting tryptophan from fermentation broth
  • A method for extracting tryptophan from fermentation broth
  • A method for extracting tryptophan from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: A kind of method for extracting tryptophan from fermented liquid, present embodiment does not add crystallization auxiliary agent, concrete steps are:

[0042] After the fermentation finishes, get 30L fermented liquid immediately, the L-tryptophan content in the fermented liquid is 51.6g / L, control stirring speed to be 100 revs / min, adjust the pH value of fermented liquid to carry out isoelectric crystallization, specifically: Add sulfuric acid to the fermentation broth to adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 drop every 5 minutes. After reaching the pH, maintain it for 15 minutes. The isoelectric crystallization temperature is 40°C; To reduce the temperature by 3°C per hour, the temperature was lowered to 10°C and maintained for 30 minutes. Use an automatic slag removal filter to filter and separate the crystals, add 6L of ethanol with a mass volume ratio of 10% to rinse before the end of the filtration, and obtain 986g of ...

Embodiment 2

[0049] Embodiment 2: a method for extracting tryptophan from fermentation broth, this embodiment adds 100ppm of crystallization aid, and the specific steps are:

[0050] After the fermentation is finished, take 30L of fermented liquid immediately, the content of L-tryptophan in the fermented liquid is 50.4g / L, control the stirring speed to be 100 revs / min, add 100ppm fatty acid glyceride, adjust the pH value of the fermented liquid for isoelectric Crystallization, specifically: add sulfuric acid to the fermentation broth, adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 drop every 5 minutes, maintain the pH for 15 minutes after the pH is reached, and the isoelectric crystallization temperature is 40°C; after isoelectric crystallization Start cooling crystallization, the cooling gradient is 3 ° C per hour, the temperature drops to 10 ° C and maintains for 30 minutes. Use an automatic slag removal filter to filter and separate the crystals, add 6L of ethanol...

Embodiment 3

[0057] Embodiment 3: A kind of method for extracting tryptophan from fermented liquid, this embodiment adds crystallization auxiliary agent 250ppm, concrete steps are:

[0058] After the fermentation is finished, take 30L of fermented liquid immediately, the content of L-tryptophan in the fermented liquid is 52.6g / L, control the stirring speed to be 200 revs / min, add 250ppm fatty acid glyceride, adjust the pH value of the fermented liquid for isoelectric Crystallization, specifically: add sulfuric acid to the fermentation broth, adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 drop every 5 minutes, maintain the pH for 15 minutes after the pH is reached, and the isoelectric crystallization temperature is 38°C; after isoelectric crystallization Start cooling crystallization, the temperature drop gradient is 3 ° C per hour, until the temperature is 15 ° C, and maintained for 30 minutes. Use an automatic slag removal filter to filter and separate the crystals,...

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Abstract

The invention discloses a method for extracting tryptophan from fermentation broth, which comprises the following steps: (1) primary crystallization of fermentation broth; (2) separation of primary crystals; (3) secondary crystallization of fermentation broth; (4) Obtain the finished product of tryptophan. The present invention firstly separates the primary crystal from the tryptophan fermentation liquid, then removes the bacteria and protein in the fermentation liquid, evaporates and concentrates the obtained clear liquid after decolorization, crystallizes and separates to obtain the secondary crystal, completely without separation process and less sewage discharge , friendly to the environment; process without organic film, less investment in equipment, low cost. In the case of reducing costs, water resources and heat energy consumption, the product purity and yield are greatly improved compared with the existing process; the process of the invention is suitable for large-scale industrial production.

Description

Technical field: [0001] The invention relates to a production method of tryptophan, in particular to a method for extracting tryptophan from fermentation broth. Background technique: [0002] As an essential amino acid for human and animal life activities, tryptophan is widely used in food, feed, medicine, agriculture and forestry and other industries. Adding tryptophan to food can promote protein absorption by the body. As a feed additive, adding tryptophan to low-protein diets is very effective in increasing the weight gain rate of livestock and poultry and improving feed efficiency. Tryptophan is the fourth largest feed addition after lysine, threonine and methionine amino acid. The metabolite of tryptophan, 5-hydroxytryptophan, has anti-stress effects such as high density and weaning in animals, and can promote the production of R-globulin and enhance the body's disease resistance. In addition, tryptophan also affects the behavior of livestock and poultry, which can r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 吴涛王海雷常利斌龚华李岩赵津津刘月明
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD