A kind of proliferation medium formula and application of cultivating stout tetraploid paulownia seedlings

A proliferation medium and tetraploid technology, applied in the biological field, can solve the problems of reducing the survival rate of transplanted seedlings, weak growth of adventitious buds, and high multiplication coefficient, so as to improve the survival rate of transplanted seedlings and solve the serious shortage of supply , the effect of promoting proliferation

Active Publication Date: 2021-05-18
SHANDONG FOREST SCI RES INST +2
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the multiplication coefficient is too high, there are too many clustered buds induced, and most of the adventitious buds grow weaker. If the formula is not adjusted in time in the next culture cycle, after these weak regenerated shoots are transferred to the rooting medium, the growth will be reduced. Weaker, resulting in reduced survival rate of transplanted seedlings

Method used

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  • A kind of proliferation medium formula and application of cultivating stout tetraploid paulownia seedlings
  • A kind of proliferation medium formula and application of cultivating stout tetraploid paulownia seedlings
  • A kind of proliferation medium formula and application of cultivating stout tetraploid paulownia seedlings

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Experimental program
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Effect test

Embodiment 1

[0024]Cut the aseptic seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating stout tetraploid Paulownia seedlings MS+IAA 0.05mg / L+6-BA 0.5mg / L+KT 0.05mg / L +NAA 0.05mg / L+(NH4)2HPO4 50mg / L +agar 4.5g / L+sucrose 30g / L (pH 5.8), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d. After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 3-5 times (see figure 1 ).

Embodiment 2

[0026] Cut the aseptic seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating strong tetraploid paulownia seedlings MS+IAA 0.5mg / L+6-BA 3.0mg / L+KT 1.0mg / L +NAA 1.0mg / L+(NH4)2HPO4 200mg / L+ agar 5.0g / L+ sucrose 30g / L (pH 5.8), culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d . After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 5-8 times (see figure 2 ).

Embodiment 3

[0028] Cut the sterile seedlings into about 2cm long bud stem segments, and transfer them to the proliferation medium for cultivating strong tetraploid Paulownia seedlings MS+IAA 3.0mg / L+6-BA 7.0mg / L+KT 3.0mg / L +NAA 3.0mg / L+(NH4)2HPO4 500mg / L+ agar 6.0g / L+ sucrose 40g / L (pH 6.0), the culture conditions are daytime temperature 25°C, night temperature 18°C, light intensity 2500Lx, light time 13h / d . After 20 days, induce to form thick and strong adventitious buds, and the multiplication factor is 3-6 times (see image 3 ).

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Abstract

The invention discloses a formula and application of a proliferation medium for cultivating robust tetraploid paulownia seedlings. It consists of MS medium supplemented with 0.05‑3.0mg / L indoleacetic acid, 0.5‑7.0mg / L 6‑benzylaminoadenine, 0.05‑3.0mg / L kinetin, 0.05‑3.0mg / L naphthaleneacetic acid, 50‑500mg / L diammonium hydrogen phosphate, 4.0‑6.0g / L agar, 30‑40g / L sucrose. This formula can significantly promote the adventitious buds regenerated from tetraploid Paulownia to be strong and high-quality, with an expansion coefficient of 3‑9, easier to take root, improve the survival rate of transplanted and hardened seedlings, and provide technical support for the factory production of excellent tetraploid Paulownia strains. Solve the problem that the supply of tetraploid Paulownia seedlings is seriously in short supply.

Description

technical field [0001] The invention relates to a formula and application of a proliferation medium for cultivating robust tetraploid paulownia seedlings, which is especially suitable for subgeneration and multiplication in the later stage of industrialized seedling cultivation, and belongs to the field of biotechnology. Background technique [0002] Paulownia is a plant of the genus Paulownia in the family Scrophulariaceae. It is native to my country. It has no flying catkins, beautiful trees, and a long history of cultivation. It is naturally distributed in 25 provinces, municipalities, and autonomous regions in my country. It is an important fast-growing timber and native tree species for landscaping in my country. The leaves, flowers, fruits and bark of Paulownia can be used as medicine, and the leaves and flowers of Paulownia can be used as feed. Paulownia wood is high-quality and high-grade, corrosion-resistant, acid and alkali resistant, wear-resistant, easy to proces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 毛秀红闫少波王迎毛欣刘泉华辉亓玉昆仲伟国李猛张新慰张倩王晓英
Owner SHANDONG FOREST SCI RES INST
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