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Pharmaceutical use of harmine

A technology of dehydrohalamine and medicine, which is applied in the field of medicine and can solve the problems of no dehydrohalamine, etc.

Inactive Publication Date: 2020-07-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the induction of endogenous cardiomyocyte proliferation by dehydrohampine

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the in vitro test that dehydrohaemaline promotes the proliferation of rat cardiomyocytes

[0030] (1) Culture of Cardiomyocytes of SD Rats

[0031] Cardiomyocytes from SD rats born 3 days old were isolated and cultured in DMEM high glucose medium (Hyclone) + 5% horse serum (GIBCO) at 37 degrees in a 5% carbon dioxide incubator.

[0032] (2) Experimental grouping and processing

[0033] Isolate cardiomyocytes from SD rats, add 5% horse serum (GIBCO) + DMEM high-glucose medium (Hyclone), and add cytarabine (final concentration 20umol / L) to inhibit the growth of non-cardiomyocytes. Infect cTnT-mAG-hGeminin (1 / 110) virus (MOI value of virus infection=100) after 48 hours of adherence, after another 24 hours, replace with DMEM culture medium containing 0.5% FBS, add drugs in groups, and group as follows:

[0034] a. Experimental group: treated with Harmine small molecule (final concentration in the culture medium is 5 μmol / l) for 24 hours.

[0035] b. Blank c...

Embodiment 2

[0041] Example 2. In vitro test of the proliferation of cardiomyocytes (hiPSC-derived cardiomyocytes) induced by human induced pluripotent stem cells by dehydrohaeline

[0042] Obtain hiPSC-derived cardiomyocytes, add Harmine (the final concentration in the medium is 5 μmol / l) to treat for 48h, use RPMI1640+DMSO as the control (control), wash with PBS three times after 48h, fix with 4% PFA (paraformaldehyde) at room temperature 15min, wash three times with PBS, block with 1%BSA / PBS / 0.1%Triton at room temperature for 1h, add primary antibody after 1h: Ctnt (1:200, abcam, ab8295), Phospho-Histone H3 (Ser10) antibody (1:200, CST , 9701S) overnight at 4°C, wash 4 times with 1% BSA / PBS / 0.02% Tween, add secondary antibody: goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (1:300, Invitrogen, A32731) , goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (1:300, Lifetechnologies, A21424), placed at room temperature for 1 hour, washed 3 times with PBS / 0...

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PUM

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Abstract

The invention discloses a pharmaceutical use of harmine. The use refers to use of harmine or pharmaceutically acceptable salt and ester thereof in preparation of a medicine for promoting myocardial cell proliferation. Experiments prove that the harmine can promote the proliferation of rat myocardial cells, and can also promote proliferation of human induced pluripotent stem cell induced cardiomyocytes (hiPSC-derived cardiomyocytes). Therefore, the harmine can be used in relevant fields of preparing medicines for promoting myocardial cell proliferation and treating or preventing heart diseasesand the like, thereby providing a new medicine and treatment idea for treating or preventing heart diseases such as myocardial infarction.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a medicinal application of dehydrohalamine. Background technique [0002] At present, cardiovascular disease has become the number one killer threatening human health. There are 40 million heart failure patients worldwide, and it has become the main cause of human death. Studies have found that cardiomyocytes in mammals gradually lose their ability to proliferate after adulthood. Once myocardial infarction occurs, the loss of cardiomyocytes will be irreversible. There are about 2-4 billion cardiomyocytes in an adult, about 25% of cardiomyocytes will be lost within a few hours after myocardial infarction, and the remaining cardiomyocytes have very limited proliferation ability, which is not enough to restore the contractile function of the heart, and the patient will eventually suffer from heart failure die. To solve this problem, in addition to surgery, basic translational r...

Claims

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Application Information

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IPC IPC(8): A61K31/437A61P9/00
CPCA61K31/437A61P9/00
Inventor 熊敬维杜建勇郑丽霞朱小君
Owner PEKING UNIV
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