Check patentability & draft patents in minutes with Patsnap Eureka AI!

Application of CPSF4 autoantibody detection reagent in preparation of lung cancer screening kit

A CPSF4, autoantibody technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of no lung cancer, no CPSF4 protein, etc.

Pending Publication Date: 2020-07-07
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on CPSF4 protein autoantibodies, and there is no prior art related to lung cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CPSF4 autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of CPSF4 autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of CPSF4 autoantibody detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Relationship between CPSF4 Autoantibodies in Plasma and Lung Cancer

[0027] 1. Clinical data

[0028] 30 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0029] Basic Information lung cancer patients healthy control number of people 30 29 age 58±10.5 42±8.9 male ratio 20(66.7％) 13(44.8％)

[0030] 2. Detection principle

[0031] HuProt TM CPSF4 protein (the CPSF4 protein used is a full-length protein, UniProtKB number: O95639) is immobilized on the human protein custom chip. After incubation with serum, CPSF4 autoantibodies (mainly including IgG and IgM antibodies, and some other types Antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then use anti-human IgM fluorescently labeled secondary antibody (cy5 labeled, red) and anti-human IgG fluorescent secondary antibody (cy3 labeled, green) to detect, through fluorescence The scanner re...

Embodiment 2

[0048] Embodiment 2 The composition of detection kit of the present invention and its application method

[0049] 1. Kit composition

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to an application of a CPSF4 autoantibody detection reagent in preparation of a lung cancer screening kit. It is foundfor the first time that the autoantibody level of CPSF4 protein in serum of a lung cancer patient is remarkably lower than that of CPSF4 protein in healthy people. The reagent for detecting the CPSF4protein autoantibody is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of a CPSF4 autoantibody detection reagent in the preparation of a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung canc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488
Inventor 王巍
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com