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Method for Inducing Chromosome Ploidy of Mulberry Seedlings Using Cotton Tubes

A chromosome ploidy, cotton tube technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of low induction effect, high labor intensity, heavy air pollution, etc. The effect of high work efficiency and accurate dripping target

Active Publication Date: 2022-02-01
广西壮族自治区蚕业技术推广站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Still another object of the present invention is to provide a method for inducing chromosome ploidy of mulberry seedlings using a cotton tube, which can effectively solve the traditional method of long time for dripping medicine, high labor intensity, high labor cost, low induction effect and serious air pollution. and other problems, the waste of absorbent cotton and mutagen has also been fundamentally curbed; in addition, using the corrective effect of the support rods, the original oblique or curved seedlings can be used as effective induction seedlings, which significantly improves resource utilization; and The method of the present invention uses the cotton tube to directly cover the bud eye and the two sides of the inner cavity are embedded with sawtooth, which not only fixes the position of the cotton wool body, isolates the surrounding air circulation, directly reduces the volatilization of chemical agents, but also reduces pollution, ensuring the induced Effect

Method used

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  • Method for Inducing Chromosome Ploidy of Mulberry Seedlings Using Cotton Tubes
  • Method for Inducing Chromosome Ploidy of Mulberry Seedlings Using Cotton Tubes
  • Method for Inducing Chromosome Ploidy of Mulberry Seedlings Using Cotton Tubes

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Experimental program
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Effect test

Embodiment 1

[0037] Such as Figure 1-4 Shown, a kind of method utilizing cotton tube to carry out mulberry seedling chromosome ploidy induction, it may further comprise the steps:

[0038] 1) Raising mulberry seedlings, 3 days after emergence, when the two leaves 5 are fully unfolded and the bud eyes of the true leaves are exposed, the mulberry seedlings are chemically induced;

[0039] 2) The lower end of the sleeve cotton tube is aligned with the bud eye of the mulberry seedling, and the sleeve cotton tube is fixed on the bud eye of the mulberry seedling;

[0040] Wherein, the sleeve cotton tube includes:

[0041] cotton wool;

[0042] The tube body 1 has a pair of notches 2 on the tube wall at its lower end, the cotton wool is filled in the tube body 1, and the two notches 2 are respectively clamped on the petioles of the two leaves 5 of the seedlings to fix the cotton tube on mulberry seedlings;

[0043] 3) Inducing agents are dripped into the cotton wool, planted continuously, an...

Embodiment 2

[0045] Such as Figure 1-4 Shown, a kind of method utilizing cotton tube to carry out mulberry seedling chromosome ploidy induction, it may further comprise the steps:

[0046] 1) Raising mulberry seedlings, 5 days after emergence, when the two leaves 5 are fully unfolded and the bud eyes of the true leaves are exposed, the mulberry seedlings are chemically induced;

[0047] 2) The lower end of the sleeve cotton tube is aligned with the bud eye of the mulberry seedling, and the sleeve cotton tube is fixed on the bud eye of the mulberry seedling;

[0048] Wherein, the sleeve cotton tube includes:

[0049] cotton wool;

[0050]The tube body 1 has a pair of notches 2 on the tube wall at its lower end, the cotton wool is filled in the tube body 1, and the two notches 2 are respectively clamped on the petioles of the two leaves 5 of the seedlings to fix the cotton tube on mulberry seedlings;

[0051] It also includes two support rods 3, which are arranged at the lower end of th...

Embodiment 3

[0054] Such as Figure 1-4 Shown, a kind of method utilizing cotton tube to carry out mulberry seedling chromosome ploidy induction, it comprises the following steps:

[0055] 1) For mulberry seedling cultivation, when the two leaves 5 are fully unfolded for 4 days after emergence and the bud eyes of the true leaves are exposed, the mulberry seedlings are chemically induced;

[0056] 2) The lower end of the sleeve cotton tube is aligned with the bud eye of the mulberry seedling, and the sleeve cotton tube is fixed on the bud eye of the mulberry seedling;

[0057] Wherein, the sleeve cotton tube includes:

[0058] cotton wool;

[0059] The tube body 1 has a pair of notches 2 on the tube wall at its lower end, the cotton wool is filled in the tube body 1, and the two notches 2 are respectively clamped on the petioles of the two leaves 5 of the seedlings to fix the cotton tube on mulberry seedlings;

[0060] It also includes two support rods 3, which are arranged at the lower...

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Abstract

The invention discloses a method for inducing chromosome ploidy of mulberry seedlings by using a cotton tube. Sang seedlings are chemically induced; 2) the lower end of the sleeve cotton tube is aligned with the bud eyes of mulberry seedlings, and the sleeve cotton tube is fixed on the bud eyes of mulberry seedlings; the sleeve cotton tube includes: cotton wool; tube body, the tube wall at its lower end A pair of gaps are arranged on the top, and the cotton wool is filled in the tube body, and the two gaps are respectively clamped on the petioles of the two leaves of the seedlings to fix the cotton tube on the mulberry seedlings; 3) drop the cotton wool into the Inducer, continuous planting, and identification of chromosome ploidy, to obtain successfully induced mulberry seedlings. The method of the present invention has the characteristics of simple operation, greatly reducing the time for operators to be in contact with chemical inducers, dropping medicine to the cotton wool once a day to keep the cotton cotton moist throughout the day, improving the success rate of induction of mulberry seedlings and correcting the growth of curved seedlings, etc. .

Description

technical field [0001] The invention relates to a method for inducing polyploidy of mulberry trees. More specifically, the present invention relates to a method for inducing chromosome ploidy of mulberry seedlings by using a cotton tube. Background technique [0002] Mulberry mutagenesis is often carried out in the process of breeding of improved varieties and innovation of germplasm resources. Among them, chemical mutagenesis is the fastest and most direct method. The principle is to rely on the action of chemical mutagens to induce mutations in plant genetic material, thereby causing Variation in plant appearance and morphological characteristics. Therefore, the use of chemical mutagens for mulberry mutagenesis is the most common and frequently used method in germplasm resource innovation research. In the actual operation of chemical mutagenesis of mulberry trees, the method is single, time-consuming and labor-intensive. The medication operation process is to roll up abs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01G17/00
CPCA01H1/08A01G17/005
Inventor 崔秋英邱长玉林强朱方容石华月朱光书张朝华陈芳林强轩唐燕梅李韬卢德
Owner 广西壮族自治区蚕业技术推广站