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The invention also discloses application of UTIMP-2 and IGFBP-7 in preparation of biomarker for diagnosing sepsis acute kidney injury

A 2. IGFBP-7, IGFBP-7 technology, applied in the field of bioengineering, can solve the problems of biomarker hysteresis, unable to accurately reflect kidney damage in an early stage, and achieve the effect of significant technological progress and solving the problem of delayed diagnosis

Pending Publication Date: 2020-07-14
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0003] The purpose of the present invention is to provide the use of urinary TIMP-2 and IGFBP-7 in the preparation of biomarkers for the diagnosis of acute kidney injury in sepsis. The use of biomarkers for diagnosing sepsis-acute kidney injury is to solve the technical problem that the biomarkers used for diagnosing sepsis-acute kidney injury in the prior art have hysteresis and cannot accurately reflect the occurrence of kidney injury in the early stage

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  • The invention also discloses application of UTIMP-2 and IGFBP-7 in preparation of biomarker for diagnosing sepsis acute kidney injury
  • The invention also discloses application of UTIMP-2 and IGFBP-7 in preparation of biomarker for diagnosing sepsis acute kidney injury
  • The invention also discloses application of UTIMP-2 and IGFBP-7 in preparation of biomarker for diagnosing sepsis acute kidney injury

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Embodiment 1

[0019] We selected 85 patients with sepsis who were admitted to the emergency ICU and comprehensive ICU of Shanghai Dongfang Hospital from November 2017 to December 2019. According to the KIDGO diagnostic criteria, they were divided into AKI group with 37 cases and non-AKI group with 48 cases. Healthy controls were also set up. Group of 20 cases. 10ml of urine samples were collected from patients with sepsis at 0 hour, 6 hours, 12 hours, 1 day, 3 days, and 7 days, and the levels of urinary TIMP-2 and IGFBP-7 at each time point were detected by enzyme-linked immunosorbent assay (ELISA). Level, draw the receiver operating characteristic (ROC) curve, and calculate the area under the curve (AUC). The study found that the levels of urinary TIMP-2 and IGFBP-7 in the sepsis AKI group were significantly higher than those in the control group, while there was no significant difference in the sepsis non-AKI group. The levels of urinary TIMP-2 and IGFBP-7 in the AKI group were significa...

Embodiment 2

[0027] Example 2 ROC curve analysis and combined diagnostic value of urinary TIMP-2 and IGFBP-7 at each time point in AKI group

[0028] The ROC curve showed that the area under the curve of urinary TIMP-2 at each point was 0.627, 0.642, 0.734, 0.947, 0.714 and 0.543, and the area under the curve reached a peak value of 0.947 at 1 day. The sensitivity and specificity were respectively 97.5%, 81.2%, the cut-off value is 151.23ng / ml ( image 3). The area under the curve of urinary IGFBP-7 at each point was 0.754, 0.958, 1, 0.860, 0.701 and 0.707, and the area under the curve reached a peak value of 1 at 12 hours. The sensitivity and specificity were 98.9%, 72.8%, respectively, The cutoff value is 14.91ng / ml ( Figure 4 ). The area under the curve of joint detection of urinary TIMP-2×IGFBP-7 at each point was 0.873, 0.988, 1.00, 0.953, 0.730 and 0.814, and the area under the curve of joint detection of urinary TIMP-2×IGFBP-7 at 12 hours The area under the curve reached the pe...

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Abstract

The invention provides an application of urine TIMP-2 in preparation of a biomarker for diagnosing sepsis acute kidney injury. The invention also provides application of IGFBP-7 in preparation of a biomarker for diagnosing sepsis acute kidney injury. The invention further provides application of the urine TIMP-2 combined with IGFBP-7 in preparation of a biomarker for diagnosing sepsis acute kidneyinjury. The invention further provides a kit for detecting acute kidney injury caused by sepsis diagnosis. The kit contains reagents for detecting TIMP-2 and IGFBP-7 in a body fluid sample. It is found that the content of urine IGFBP-7 and TIMP-2 in clinical sepsis AKI patients is remarkably higher than that of urine IGFBP-7 and TIMP-2 in health examination and non-AKI group patients; through ROCcurve analysis, it is found that urine IGFBP-7 and TIMP-2 have high benefits in diagnosis of acute kidney injury caused by sepsis as molecular biomarkers.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a biomarker, specifically the use of urinary TIMP-2 and IGFBP-7 in preparing biomarkers for diagnosing sepsis acute kidney injury. Background technique [0002] Sepsis is a life-threatening organ dysfunction caused by an uncontrolled host response to infection. Despite the continuous improvement of treatment methods, the current mortality rate is still as high as 40%. Among the organ dysfunctions induced by sepsis, the lungs and kidneys are the most common. Studies have shown that the incidence of sepsis complicated with acute kidney injury (acute kidney injury, AKI) is as high as 44.9%, and the case fatality rate is close to 50%. Currently, the diagnostic criteria for sepsis AKI are mainly RIFLE (risk, injury, failure, loss, end stage kidney disease) and KIDGO (kidney disease: improving global outcomes organization) criteria, and the main indicators are still glomerular filtration ra...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/347G01N2800/26G01N2333/65G01N2333/8146
Inventor 唐伦先任慧娟杨倩赵冬旸刘显东杨杏静黄伟琴吕迪宇李秀华白建文
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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