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Hepatitis B virus X gene and application thereof

A hepatitis B virus and gene technology, applied in the medical field, can solve the problems of restricting the clinical development of PH and reducing the ability of liver regeneration.

Inactive Publication Date: 2020-07-17
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liver fibrosis and cirrhosis can significantly reduce the ability of liver regeneration, leading to as high as 15% of liver failure after PH, which severely limits the clinical development of PH.

Method used

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  • Hepatitis B virus X gene and application thereof
  • Hepatitis B virus X gene and application thereof
  • Hepatitis B virus X gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The present invention will be described in detail below in combination with specific embodiments.

[0027] Implementation steps

[0028] Step 1. Collection and preparation of serum samples;

[0029] Step 2, extracting viral genomic DNA segments from serum samples of patients with HBV-related liver cancer;

[0030] Step 3, nested PCR amplification of HBx gene;

[0031] Step 4, agarose gel electrophoresis;

[0032] Step 5, gel recovery PCR product;

[0033] Step 6, sequencing of HBx fragments;

[0034] Step 7, sequencing sequence analysis;

[0035] Step 8. XXDFY in the training group predicts the prognosis of patients with liver cancer after PH

[0036] Step 9. In the verification group, verify the indication effect of XXDFY on the prognosis of patients with liver cancer after PH

[0037] Step 10. Determine the effect of XXDFY on liver regeneration in transgenic mice

[0038] Step 11. Determine the effect of XXDFY on liver regeneration in patients with liver cance...

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PUM

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Abstract

The invention discloses a hepatitis B virus X gene and application thereof. The X gene comprises an X gene sequence XXDFY. The X gene sequence XXDFY can be used for preoperatively judging the prognosis and liver regeneration condition of a progressive liver cancer patient after hepatectomy. After partial hepatectomy, the tumor-free survival rate and the overall survival rate of patients with livercancer carrying XXDFY subtype in peripheral blood are significantly higher than that of patients without XXDFY subtype.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to the application of a hepatitis B virus (HBV) X gene XDFY subtype (XXDFY) and serum XXDFY before partial hepatectomy (PH) to evaluate liver regeneration after surgery for advanced primary liver cancer and prognostic methods. HBV: hepatitis B virus, XXDFY: X gene XDFY subtype. Background technique [0002] Primary hepatocellular carcinoma (hereinafter referred to as liver cancer) is one of the most common clinical malignant tumors, and its case fatality rate ranks second in the world. Liver cancer is highly prevalent in my country. At present, the incidence of liver cancer in my country accounts for about 55% of the world's total, ranking second only to lung cancer in tumor-related deaths. Therefore, liver cancer seriously threatens the health and life of our people. Unlike alcoholic cirrhosis caused by excessive drinking in European and American countries, which is the main caus...

Claims

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Application Information

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IPC IPC(8): C12N15/51C12Q1/70C12Q1/6869C12R1/93
CPCC07K14/005C12N2730/10122C12Q1/6869C12Q1/706C12Q2531/113C12Q2549/119
Inventor 徐庆国臧运金朱晓丹许传屾戴德淑张勇孙延东
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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