Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Synthesis method of Systemic Lupus Erythematosus (SLE) treatment nanoparticles

A nanoparticle, lupus erythematosus technology, used in drug combinations, pharmaceutical formulations, allergic diseases, etc., can solve problems such as side effects and unsatisfactory curative effects, and achieve good biocompatibility, good encapsulation and film formation. performance effect

Inactive Publication Date: 2020-07-28
TIANJIN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficacy of this therapy is sometimes unsatisfactory, and continued use can cause severe side effects, highlighting the need to explore a highly effective, safe and better treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthesis method of Systemic Lupus Erythematosus (SLE) treatment nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Weigh 10 mg of PLGA material, add 20 ml of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 0.5 mg / ml;

[0023] 2) Weigh 10 mg of PEG material, add 20 ml of water and ultrasonically dissolve to obtain a PEG aqueous solution with a concentration of 0.5 mg / ml;

[0024] 3) Weigh 20 mg of IL-10 material, add 20 ml of dichloromethane and dissolve it ultrasonically to obtain an IL-10 organic solution with a concentration of 1 mg / ml;

[0025] 4) Synthesis of HIV-Vpu protein@IL-10@PEG-PLGA nanoparticles by thin film hydration method.

[0026] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 12min, the temperature to 0°C, and the ultrasonic frequency to 4s, 2s;

[0027] (2) Add 100uL of IL-10 solution and 2ml of PEG aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0028] (3) The product that ...

Embodiment 2

[0031] 1) Weigh 5 mg of PLGA material, add 20 ml of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 0.25 mg / ml;

[0032] 2) Weigh 5 mg of PEG material, add 20 ml of water and ultrasonically dissolve to obtain a PEG aqueous solution with a concentration of 0.25 mg / ml;

[0033] 3) Weigh 10 mg of IL-10 material, add 20 ml of dichloromethane and ultrasonically dissolve it to obtain an IL-10 organic solution with a concentration of 0.5 mg / ml;

[0034] 4) Synthesis of HIV-Vpu protein@IL-10@PEG-PLGA nanoparticles by thin film hydration method.

[0035] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 12min, the temperature to 0°C, and the ultrasonic frequency to 4s, 2s;

[0036] (2) Add 100uL of IL-10 solution and 2ml of PEG aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0037] (3) The product tha...

Embodiment 3

[0040] 1) Weigh 20 mg of PLGA material, add 20 ml of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 1 mg / ml;

[0041] 2) Weigh 20mg of PEG material, add 20ml of water and ultrasonically dissolve to obtain a PEG aqueous solution with a concentration of 1mg / ml;

[0042] 3) Weigh 30 mg of IL-10 material, add 20 ml of dichloromethane and dissolve it ultrasonically to obtain an IL-10 organic solution with a concentration of 1.5 mg / ml;

[0043] 4) Synthesis of HIV-Vpu protein@IL-10@PEG-PLGA nanoparticles by thin film hydration method.

[0044] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 12min, the temperature to 0°C, and the ultrasonic frequency to 4s, 2s;

[0045] (2) Add 100uL of IL-10 solution and 2ml of PEG aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0046] (3) The product that has b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a synthesis method of Systemic Lupus Erythematosus (SLE) treatment nanoparticles. The synthesis method comprises the main steps of: 1, preparing PEG aqueous solution; 2, preparing PLGA organic solution; and 3, synthesizing IL-10@HIV-Vpu protein nanoparticles by a filming-rehydration method, wherein an auxiliary protein U (Vpu) can inhibit activation of a transcription factor referred to as NF-kB, reduces generation of a cell factor taking the key effect in the immunoreaction, and inhibits a body immunity function. Interleukin-10 (IL-10) is a cell factor with multiple cell sources and multiple functions, regulates growth and differentiation of cells, participates in an inflammatory reaction and immunoreaction, and is a currently acknowledged inflammation and immunityinhibition factor. PLGA-PEG is non-toxic, has good biocompatibility, and has excellent encystation and film forming properties.

Description

technical field [0001] The present invention relates to the technical field of nanoparticle synthesis, in particular to a strategy for encapsulating HIV-Vpu protein and interleukin-10 (IL-10) with PLGA-PEG to synthesize HIV-Vpu protein@IL-10@PEG-PLGA nano preparations Methods. Background technique [0002] Systemic lupus erythematosus (SLE) involves complex pathological mechanisms, causing multi-organ damage and may be life-threatening. Nonspecific immunosuppressive or anti-inflammatory drugs, such as glucocorticoids (GCs), cyclophosphamide, and methotrexate, are commonly used in SLE. However, the efficacy of this therapy is sometimes unsatisfactory, and continuous use can cause serious side effects, which highlights the need to explore a highly effective, safe and better treatment. HIV produces a variety of proteins that play a role in suppressing the immune response. Among them, accessory protein U (Vpu), which inhibits the activation of a transcription factor called NF...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K38/20A61K38/16A61P37/06
CPCA61K9/5146A61K38/2066A61K38/162A61P37/06
Inventor 郑斌彭文畅明东甘霖
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com