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Transgenic chlamydomonas used for increasing lutein content in chlamydomonas reinhardtii, and construction method and application thereof

A technology for algal lutein and a construction method, applied in the field of biological genetic engineering, can solve problems such as unfavorable transformation to improve lutein yield, instability of the genetic transformation system of Chlorella vulgaris, etc., and achieve low cost, rapid acquisition, and rapid growth. Effect

Active Publication Date: 2020-07-28
SHENZHEN UNIV
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Problems solved by technology

[0004] At present, the methods for improving the extraction of lutein from Chlorella vulgaris mainly include optimizing the extraction method and optimizing the culture conditions. Due to the instability of the genetic transformation system of Chlorella vulgaris, it is not conducive to improving the yield of lutein through genetic engineering.

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  • Transgenic chlamydomonas used for increasing lutein content in chlamydomonas reinhardtii, and construction method and application thereof
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  • Transgenic chlamydomonas used for increasing lutein content in chlamydomonas reinhardtii, and construction method and application thereof

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[0031] The present invention provides transgenic Chlamydomonas and its construction method for increasing the lutein content of Chlamydomonas reinhardtii. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described with reference to the accompanying drawings and examples. Detailed description. It should be understood that the specific embodiments described herein are only

[0032] It is used to explain the present invention, not to limit the present invention.

[0033] Those skilled in the art can understand that unless otherwise stated, the singular forms "one", "one" and

[0034] "A", "the" and "the" may also include plural forms. It should be further understood that the word "comprising" used in the description of the present invention refers to the presence of said features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of o...

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Abstract

The invention provides transgenic chlamydomonas used for increasing the lutein content in chlamydomonas reinhardtii, and a construction method and application thereof. The construction method comprises the steps of: performing amplification to obtain lycopene epsilon-cyclase genes of chlorella vulgaris, and constructing a lycopene epsilon-cyclase gene recombination expression vector; and, converting the lycopene epsilon-cyclase gene recombination expression vector into chlamydomonas reinhardtii cells, so that transgenic lycopene epsilon-cyclase gene chlamydomonas reinhardtii is obtained. The obtained transgenic chlamydomonas can realize high yield of lutein; and furthermore, by utilization of the characteristics of vegetative propagation and rapid growth of algas, the lutein can be rapidlyobtained in low cost.

Description

technical field [0001] The invention relates to the technical field of biological genetic engineering, in particular to a transgenic Chlamydomonas for increasing the lutein content of Chlamydomonas reinhardtii and its construction method and application. Background technique [0002] The most notable biological activity of lutein is its antioxidant properties. Lutein can be rapidly oxidized by oxidants, thereby reducing the probability of intracellular free radicals and other substances being oxidized, thereby achieving antioxidant protection for the body. Similar to other carotenoid members, lutein owes its antioxidant capacity to its conjugated carbon double bond system. This structure allows quenching of singlet oxygen and scavenging of free radicals. Because of its powerful antioxidant properties, it is a class of nutrients that maintains and promotes good health, and mounting evidence supports their protective role in preventing or delaying chronic disease. [0003] ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/61C12N15/82C12N15/66C12N1/13C12N9/90C12R1/89
CPCC12N9/90C12N15/82C12N15/66C12Y505/01018
Inventor 娄素琳胡章立黄思敏林鑫
Owner SHENZHEN UNIV
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