Microscope multi-view automatic focusing system and using method thereof
An autofocus and microscope technology, applied in the field of digital images, can solve the problems of lack of dynamic focus and affecting sample integrity, etc., and achieve the effect of improving measurement integrity, saving manpower and material resources, and reducing the amount of calculation
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[0027] Build the entire microscope multi-view multi-image focusing system platform, including the construction of the control system, the video image acquisition system and the data processing system. Its overall system schematic diagram is as follows figure 1 As shown, the position of the stage is automatically adjusted by the xyz three-coordinate mobile station 3, and the multi-function acquisition card 5 uses the grayscale judgment function to evaluate and output the pulse sequence to the motor controller 4 according to the images collected by the camera 1 to control the three Asynchronous motor on coordinate platform 3. Place the sample to be tested on the mobile loading platform, and prepare for testing after the system parameters are debugged.
[0028] Manually roughly adjust the focus of the microscope objective lens to make the image observed at a certain position relatively clear, turn on the video image acquisition system, and turn on the system to automatically per...
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