Microscope multi-view automatic focusing system and using method thereof

An autofocus and microscope technology, applied in the field of digital images, can solve the problems of lack of dynamic focus and affecting sample integrity, etc., and achieve the effect of improving measurement integrity, saving manpower and material resources, and reducing the amount of calculation

Inactive Publication Date: 2020-07-28
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the autofocus system is also mainly based on static single images, lacking the dynamic focusing of the continuous positions of the samples, which affects the integrity of the obtained samples

Method used

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  • Microscope multi-view automatic focusing system and using method thereof
  • Microscope multi-view automatic focusing system and using method thereof
  • Microscope multi-view automatic focusing system and using method thereof

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Experimental program
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Embodiment

[0027] Build the entire microscope multi-view multi-image focusing system platform, including the construction of the control system, the video image acquisition system and the data processing system. Its overall system schematic diagram is as follows figure 1 As shown, the position of the stage is automatically adjusted by the xyz three-coordinate mobile station 3, and the multi-function acquisition card 5 uses the grayscale judgment function to evaluate and output the pulse sequence to the motor controller 4 according to the images collected by the camera 1 to control the three Asynchronous motor on coordinate platform 3. Place the sample to be tested on the mobile loading platform, and prepare for testing after the system parameters are debugged.

[0028] Manually roughly adjust the focus of the microscope objective lens to make the image observed at a certain position relatively clear, turn on the video image acquisition system, and turn on the system to automatically per...

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Abstract

The invention provides a microscope multi-view automatic focusing system. The microscope multi-view automatic focusing system comprises a microscope body, a stepping motor control system, a shooting unit and a movable object carrying platform. The using method comprises the following steps that: the objective lens of the microscope is manually adjusted to a relative focusing interval; the z-axis coordinate of an objective table is changed through a stepping motor in the interval, video image are obtained, the average values of the gray value variances of target object images at the end pointsof the moving interval are calculated and compared, optimization iteration is performed according to a golden section method to obtain the maximum value, and automatic focusing in the z-axis directionis realized; and the stepping motor controls the objective table to change in the xy-axis plane according to the s-shaped advancing direction, scanning of continuous positions on the plane is achieved, and finally scanned images are spliced. The automatic focusing system method provided by the invention has the advantages of low calculation complexity and the like, and can shoot high-resolution and large-view medical images.

Description

Technical field: [0001] The invention relates to the technical field of digital images, in particular to a microscope multi-view auto-focus system and a use method thereof. Background technique: [0002] Since the microscope was invented by the Jansen father and son in the Netherlands in 1590, it has been able to help researchers observe tiny objects invisible to the naked eye, opening the door to the microscopic world, and has always played a pivotal role in the biological world and even the entire scientific world. [0003] Objective lens focusing is an important part of the microscope observation process. However, with the rapid development of science and technology, the traditional manual focusing method is difficult to meet the requirements of modern science. Researchers tend to deviate the final measurement results through naked eye observation and work fatigue, and manual focusing also has defects such as low efficiency. The use of microscope autofocus technology can...

Claims

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Application Information

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IPC IPC(8): G02B7/38G02B21/36
CPCG02B7/38G02B21/365G02B21/362
Inventor 王建立陈璐张怡
Owner SOUTHEAST UNIV
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