Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for regulating and controlling CPEB3 gene expression

A technology of gene expression and culture medium, which is applied in genetic engineering, biochemical equipment and methods, and determination/inspection of microorganisms, etc.

Pending Publication Date: 2020-07-31
王亚帝
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The CPEB protein family plays an important role in the occurrence and development of various diseases by regulating various signaling pathways, such as synaptic transmission, hippocampus-dependent neurological dysfunction, colon cancer and cervical cancer, and its different family members and their regulation The miRNAs involved can be used as new targets for disease diagnosis and treatment, but the specific molecular biological mechanism involved in the cardiotoxicity of anthracyclines remains to be further studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for regulating and controlling CPEB3 gene expression
  • Method for regulating and controlling CPEB3 gene expression
  • Method for regulating and controlling CPEB3 gene expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] The invention provides a method for regulating CPEB3 gene expression, determines the target regulation relationship between hsa-miR-4638-3p and CPEB3, and lays an experimental foundation for further research on their cardiotoxicity and molecular mechanism of anthracyclines.

[0064] In view of the important role of cytoplasmic poly(A)denylation elementbinding protein (CPEB) in the growth and development of cells, the present invention uses the firefly / renilla dual-luciferase reporter system for target verification, To determine whether there is an exact target relationship between hsa-miR-4638-3p and CPEB3 gene.

[0065] 1. Materials and methods

[0066] 1.1 Using bioinformatics software to predict the sequence of CPEB3 3'UTR:

[0067] Three microRNA prediction software, TargetScan Human 7.1, miRanda, and miRDB, were used to predict. Both TargetScan Human 7.1 and miRDB predicted that hsa-miR-4638-3p could bind to CPEB3 3'UTR; miRanda database did not include miR-4638- ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for regulating and controlling CPEB3 gene expression, and relates to the technical field of biology. The method for regulating and controlling CPEB3 gene expression comprises the following steps of 1, predicting a CPEB 3 3' UTR sequence by bioinformatics software; predicting that hsa-miR-4638-3p can be combined to the CPEB3 3' UTR sequence; 2, building a CPEB3 dual-luciferase report vector; 3, performing co-transfection on hsa-miR-4638-3p and plasmid DNA finally extracted in the step 2; 4, performing luciferase detection on the co-transfection cells in the step 3; and 5, performing statistical data analysis. The method for regulating and controlling CPEB3 gene expression has the advantages that the targeted regulation and control relationship of hsa-miR-4638-3p and CPEB3 is determined; the experiment foundation is laid for further studying the cardiotoxicity effect and the molecular mechanism of anthracycline.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for regulating the expression of CPEB3 gene. Background technique [0002] miRNA is a class of endogenous non-coding small RNAs with a size of about 22 nucleotides. Studies have shown that miRNA affects gene transcription, protein synthesis, etc. through the combination with its target gene mRNA, participates in many biological processes, and affects the growth and development of animals. Although, so far, scientists have discovered many miRNA molecules and have a certain understanding of their functions, but there are still many unsolved mysteries about genes and miRNA. To solve these problems, the most simple and effective way is to start with the research and discovery of more miRNAs and their target genes, take the relationship between miRNAs and their target genes as the research direction, and conduct biological function verification so that we can learn more about mi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/63C12N15/85C12N15/113C12Q1/66
CPCC12N15/63C12N15/85C12N15/113C12Q1/66C12N2310/141Y02A50/30
Inventor 王亚帝何东宁陈素贤陈书睿李琪谭琦谷泽慧
Owner 王亚帝
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com