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Application of emodin in resisting 2019-nCoV infection

A 2019-ncov, emodin technology, applied in the field of pharmacy, can solve the problem of no effective treatment drugs for diseases

Inactive Publication Date: 2020-08-04
BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no effective treatments for the disease caused by the novel coronavirus

Method used

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  • Application of emodin in resisting 2019-nCoV infection
  • Application of emodin in resisting 2019-nCoV infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The emodin used in this example was purchased from China Food and Drug Identification Institute, the product number is PCS0249.

[0019] The experimental process steps adopted in this embodiment are as follows:

[0020] (1) Dissolve 20 mg of emodin in 1 mL of DMSO to form a mother solution with a concentration of 740 μM / mL, and dilute by 10 times (ie 740, 74, 7.4, 0.74, 0.074 μM / mL) for later use. After Vero E6 cells were cultured in 96-well plates for 24 hours, xCov at MOI=10 and emodin of various concentrations were co-treated for 3 days, and then relevant indicators were detected.

[0021] (2) After collecting Vero cells, use AxyPrepTM Body FluidViral DNA / RNA Miniprep Kit (Axygen, Cat no.AP-MN-BF-VNA-250) and AxyPrepTM Multisource Total RNA MiniprepKit (Axygene, Cat no.AP-MN-MS-RNA-250G ) to extract viral RNA.

[0022] (3) Use Hifair II 1st Strand cDNA synthesis Kit for RNA reverse transcription. Referring to the instructions of the reverse transcription kit, afte...

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Abstract

The invention discloses an application of emodin in preparation of a medicine for preventing or treating 2019-nCoV infection, and belongs to the field of pharmacy. According to the invention, a novelcoronavirus is isolated and cultured, is named as xCov, is preserved in China General Microbiological Culture Collection Center, has a preservation number of CGMCC NO.19295 and a preservation date ofFebruary 14, 2020, and is named as coronavirus by taxonomy. The S protein homology of the virus and 2019-nCoV reaches 92%, and the virus is very safe and can be used for drug screening and evaluation,vaccine screening and evaluation, attenuation and inactivated vaccine preparation of the 2019-nCoV. According to the application, xCov is used as a model, and the drug emodin with an obvious inhibition effect on xCov is screened out, so that a direction and a thought are provided for treatment of 2019-nCoV.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to the application of emodin in the preparation of medicines for preventing or treating 2019-nCoV infection. Background technique [0002] 2019-nCov is a new type of coronavirus. Common signs of people infected with this coronavirus include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. There are currently no effective treatments for the disease caused by the novel coronavirus. Contents of the invention [0003] In order to solve the above technical problems, the present invention provides the application of emodin in the preparation of medicines for preventing or treating 2019-nCoV infection, wherein the emodin has the following structural formula: [0004] [0005] In the above application, the molecule of emodin is C 15 h 10 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P31/14
CPCA61K31/122A61P31/14
Inventor 徐霄龙刘清泉郭玉红范华昊童贻刚
Owner BEIJING CHINESE MEDICINE HOSPITAL AFFILIATED CAPITAL MEDICAL UNIV